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3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink

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dc.contributor.author Restan Perez, Milena
dc.contributor.author Sharma, Ruchi
dc.contributor.author Zeina Masri, Nadia
dc.contributor.author Willerth, Stephanie
dc.date.accessioned 2021-10-01T22:17:45Z
dc.date.available 2021-10-01T22:17:45Z
dc.date.copyright 2021 en_US
dc.date.issued 2021
dc.identifier.citation 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. (2021). 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules, 11(8), 1-15. https://doi.org/10.3390/biom11081250. en_US
dc.identifier.uri https://doi.org/10.3390/biom11081250
dc.identifier.uri http://hdl.handle.net/1828/13434
dc.description.abstract Current treatments for neurodegenerative diseases aim to alleviate the symptoms experienced by patients; however, these treatments do not cure the disease nor prevent further degeneration. Improvements in current disease-modeling and drug-development practices could accelerate effective treatments for neurological diseases. To that end, 3D bioprinting has gained significant attention for engineering tissues in a rapid and reproducible fashion. Additionally, using patient-derived stem cells, which can be reprogrammed to neural-like cells, could generate personalized neural tissues. Here, adipose tissue-derived mesenchymal stem cells (MSCs) were bioprinted using a fibrin-based bioink and the microfluidic RX1 bioprinter. These tissues were cultured for 12 days in the presence of SB431542 (SB), LDN-193189 (LDN), purmorphamine (puro), fibroblast growth factor 8 (FGF8), fibroblast growth factor-basic (bFGF), and brain-derived neurotrophic factor (BDNF) to induce differentiation to dopaminergic neurons (DN). The constructs were analyzed for expression of neural markers, dopamine release, and electrophysiological activity. The cells expressed DN-specific and early neuronal markers (tyrosine hydroxylase (TH) and class III beta-tubulin (TUJ1), respectively) after 12 days of differentiation. Additionally, the tissues exhibited immature electrical signaling after treatment with potassium chloride (KCl). Overall, this work shows the potential of bioprinting engineered neural tissues from patient-derived MSCs, which could serve as an important tool for personalized disease models and drug-screening. en_US
dc.description.sponsorship This work was funded by the NSERC Discovery Grant program, NSERC Idea to Innovation program, Innovate BC’s Ignite program, the Alzheimer’s Association, Canada Research Chairs and the Michael Smith Foundation for Health Research and Pacific Parkinson’s Research Institute’s Innovation to Commercialization grant. en_US
dc.language.iso en en_US
dc.publisher Biomolecules en_US
dc.subject 3D bioprinting en_US
dc.subject fibrin en_US
dc.subject small molecules en_US
dc.subject neural tissues en_US
dc.subject stem cells en_US
dc.title 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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