Enhanced pest management of the Douglas-fir tussock moth, Orgyia pseudotsugata (Lepidoptera: Lymantriidae)

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dc.contributor.author Thorne, Christine M.
dc.date.accessioned 2009-12-08T23:04:57Z
dc.date.available 2009-12-08T23:04:57Z
dc.date.copyright 2006 en
dc.date.issued 2009-12-08T23:04:57Z
dc.identifier.uri http://hdl.handle.net/1828/1961
dc.description.abstract The incidence of baculovirus disease in susceptible insect hosts may be used to estimate future host population sizes and estimate the relative defoliation of these populations for economically important insects. We developed an Enzyme-linked immunosorbent assay (ELISA) that was specific to two native baculovirus pathogens of the Douglas-fir tussock moth (DFTM), that was more sensitive and accurate (R2=0.99) than microscopic counts of virus polyhedron inclusion bodies, and more accurate than PCR or Southern hybridization for the direct detection of baculovirus disease. We also developed a field-based "dipstick" assay that was sensitive enough to detect virus disease prior to host mortality and did not require virus purification from macerated insect tissues, supporting its use for on-site field surveys. Finally, we used ELISA to establish, for the first time, a method to detect persistent sublethal baculovirus infections. Our work may contribute to more accurate understanding of the incidence and influence of persistent sublethal infections on insect hosts. en
dc.language English eng
dc.language.iso en en
dc.rights Available to the World Wide Web en
dc.subject Douglas fir en
dc.subject Douglas fir tussock moth en
dc.subject diseases and pests en
dc.subject control en
dc.subject.lcsh UVic Subject Index::Sciences and Engineering::Biology en
dc.title Enhanced pest management of the Douglas-fir tussock moth, Orgyia pseudotsugata (Lepidoptera: Lymantriidae) en
dc.type Thesis en
dc.contributor.supervisor Levin, David Bernard
dc.contributor.supervisor Otvos, Imre S.
dc.degree.department Dept. of Biology en
dc.degree.level Master of Science M.Sc. en

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