A 44K microarray dataset of the changing transcriptome in developing Atlantic salmon (Salmo salar L.)
Date
2011-03-29
Authors
Jantzen, Stuart G.
Sanderson, Dan S.
Von Schalburg, Kristian Robert
Yasuike, Motoshige
Marass, Francesco
Koop, Benjamin F.
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Background: Atlantic salmon (Salmo salar L.) is an environmentally and economically important organism and its
gene content is reasonably well characterized. From a transcriptional standpoint, it is important to characterize the
changes in gene expression over the course of unperturbed early development, from fertilization through to the
parr stage.
Findings: S. salar samples were taken at 17 time points from 2 to 89 days post fertilization. Total RNA was
extracted and cRNA was synthesized and hybridized to a newly developed 44K oligo salmonid microarray platform.
Quantified results were subjected to preliminary data analysis and submitted to NCBI’s Gene Expression Omnibus
(GEO). Data can be found under the GEO accession number GSE25938. http://www.ncbi.nlm.nih.gov/geo/query/acc.
cgi?acc=GSE25938
Conclusions: Throughout the entire period of development, several thousand genes were found to be
differentially regulated. This work represents the trancriptional characterization of a very large geneset that will be
extremely valuable in further examination of the transcriptional changes in Atlantic salmon during the first few
months of development. The expression profiles can help to annotate salmon genes in addition to being used as
references against any number of experimental variables to which developing salmonids might be subjected.
Description
BioMed Central
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Citation
Jantzen et al.: A 44K microarray dataset of the changing transcriptome in developing Atlantic salmon (Salmo salar L.). BMC Research Notes 2011 4:88.