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Bacterial source tracking and survival of Escherichia coli

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dc.contributor.author Meays, Cynthia L.
dc.date.accessioned 2006-02-10T16:59:36Z
dc.date.available 2006-02-10T16:59:36Z
dc.date.copyright 2005 en
dc.date.issued 2006-02-10T16:59:36Z
dc.identifier.uri http://hdl.handle.net/1828/57
dc.description.abstract Surface water is used for drinking by many people around the world. E. coli is the most frequently used bacterial indicator used for assessing water quality. The survival, sources, and concentrations of E. coli were examined through a series of experiments that investigated the survival of beef cattle E. coli on land and in water, and used bacterial source tracking (BST) to determine the sources of fecal contamination diurnally and annually in multiple watersheds in British Columbia. A fecal pat experiment was conducted to examine the survival of E. coli under 4 levels of solar exposure. E. coli survived longer with increasing shade. Age of fecal pats, as well as exposure to solar radiation negatively influenced the survival of E. coli. The survival of E. coli in stream water was examined in filtered and unfiltered stream water at 3 different temperatures (6, 20 and 26 ºC). There was no significant difference in the survival of E. coli in filtered versus non-filtered stream water. Lower water temperatures (6 ºC) increased the survival of E. coli. The addition of manure to the water substantially increased the nutrient concentrations and organics. BST is a rapidly growing area of research and technology development and many methods are being developed and tested. The choice of method used for BST depends on: question(s) to be answered, scale of identification needed, available expertise, cost of analysis, turnaround time, and access to facilities. The spatial, diurnal, and annual sources and concentrations of E. coli were investigated in several watersheds in British Columbia. Fecal coliforms and E. coli concentrations varied throughout the day, as well as by site, month and year. Ribotyping identified many different sources of E. coli within the watersheds. The majority of E. coli isolates classified were from wildlife sources in each watershed even though they had different land-use. en
dc.format.extent 1653675 bytes
dc.format.mimetype application/pdf
dc.language English eng
dc.language.iso en en
dc.rights Available for the World Wide Web en
dc.subject Water quality en
dc.subject E. coli en
dc.subject Bacterial source tracking en
dc.subject Ribotyping en
dc.subject Land use en
dc.subject Watershed management en
dc.subject.lcsh Biology en
dc.subject.lcsh Microbiology en
dc.subject.lcsh Environmental sciences en
dc.title Bacterial source tracking and survival of Escherichia coli en
dc.type Thesis en
dc.contributor.supervisor Mazumder, Asit
dc.contributor.supervisor Nordin, Rick
dc.degree.department Dept. of Biology en
dc.degree.level Doctor of Philosophy Ph.D. en


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