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Towards high throughput tissue engineering: development of chitosan-calcium phosphate scaffolds for engineering bone tissue from embryonic stem cells

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dc.contributor.author Ko, Junghyuk
dc.contributor.author Kolehmainen, Kathleen
dc.contributor.author Ahmed, Farid
dc.contributor.author Jun, Martin B. G
dc.contributor.author Willerth, Stephanie
dc.date.accessioned 2015-06-30T22:08:06Z
dc.date.available 2015-06-30T22:08:06Z
dc.date.copyright 2012 en_US
dc.date.issued 2012-01-01
dc.identifier.citation Ko, J. et al Towards high throughput tissue engineering. Am J Stem Cell 2012;1(1):81-89 en_US
dc.identifier.uri http://www.ajsc.us/files/AJSC1109001.pdf
dc.identifier.uri http://hdl.handle.net/1828/6283
dc.description.abstract Tissue engineering strategies have shown promise for the repair of damaged organs, including bone. One of the major challenges associated with tissue engineering is how to scale up such processes for high throughput manufacturing of biomaterial scaffolds used to support stem cell culture. Generation of certain types of 3D biomaterial scaffolds, including chitosan-calcium phosphate blends, involves a slow fabrication process followed by a lengthy required freeze drying step. This work investigates the use of automated microwave vacuum drying technology as an alternative to traditional freeze drying as a method of fabricating chitosan-calcium phosphate scaffolds for supporting embryonic stem cell cultures. Scaffolds produced using both drying techniques possess similar properties when characterized using scanning electron microscopy and this paper is the first to report that both types of these scaffolds support undifferentiated embryonic stem cell culture as well as promote stem cell differentiation into osteogenic lineages when treated with the appropriate factors. Compared to existing scaffold manufacturing processes using freeze drying, the use of microwave vacuum drying will lead to faster production times while reducing the costs, enabling high-throughput manufacturing of biomaterial scaffolds for stem cell applications. en_US
dc.description.sponsorship The authors would like to acknowledge support from their Natural Sciences and Engineering Research Council (NSERC) Discovery Grants (MBGJ and SMW) as well as an NSERC Engage Grant (MBGJ). en_US
dc.language.iso en en_US
dc.publisher American Journal of Stem Cells en_US
dc.subject Regenerative medicine en_US
dc.subject embryonic stem cells en_US
dc.subject osteogenesis en_US
dc.subject biomaterials en_US
dc.subject composites en_US
dc.title Towards high throughput tissue engineering: development of chitosan-calcium phosphate scaffolds for engineering bone tissue from embryonic stem cells en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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