Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda) larvae responding to abiotic stress

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dc.contributor.author Sutherland, Ben J.G.
dc.contributor.author Jantzen, Stuart G.
dc.contributor.author Yasuike, Motoshige
dc.contributor.author Sanderson, Dan S.
dc.contributor.author Koop, Ben F.
dc.contributor.author Jones, Simon R.M.
dc.date.accessioned 2016-06-28T19:20:42Z
dc.date.available 2016-06-28T19:20:42Z
dc.date.copyright 2012 en_US
dc.date.issued 2012-12
dc.identifier.citation Sutherland, B.J.G., Jantzen, S.G., Yasuike, M., Sanderson, D.S., Koop, B.F., & Jones, S.R.M. (2012). Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda) larvae responding to abiotic stress. Molecular Ecology, 21(24), 6000-6014. en_US
dc.identifier.uri http://dx.doi.org/10.1111/mec.12072
dc.identifier.uri http://hdl.handle.net/1828/7375
dc.description.abstract The salmon louse Lepeophtheirus salmonis is a marine ectoparasite of wild and farmed salmon in the Northern Hemisphere. Infections of farmed salmon are of economic and ecological concern. Nauplius and copepodid salmon lice larvae are free-swimming and disperse in the water column until they encounter a host. In this study, we characterized the sublethal stress responses of L. salmonis copepodid larvae by applying a 38K oligonucleotide microarray to profile transcriptomes following 24 h exposures to suboptimal salinity (30–10 parts per thousand (&)) or temperature (16–4 °C) environments. Hyposalinity exposure resulted in large-scale gene expression changes relative to those elicited by a thermal gradient. Subsequently, transcriptome responses to a more finely resolved salinity gradient between 30 & and 25 & were profiled. Minimal changes occurred at 29 & or 28 &, a threshold of response was identified at 27 &, and the largest response was at 25 &. Differentially expressed genes were clustered by pattern of expression, and clusters were characterized by functional enrichment analysis. Results indicate larval copepods adopt two distinct coping strategies in response to short-term hyposaline stress: a primary response using molecular chaperones and catabolic processes at 27 &; and a secondary response up-regulating ion pumps, transporters, a different suite of chaperones and apoptosis-related transcripts at 26 & and 25 &. The results further our understanding of the tolerances of L. salmonis copepodids to salinity and temperature gradients and may assist in the development of salmon louse management strategies. en_US
dc.description.sponsorship This research was funded by Genome British Columbia, the Province of British Columbia, the Department of Fisheries and Oceans Canada (DFO), the University of Victoria, Grieg Seafood, Mainstream Canada and Marine Harvest. BJGS was supported by fellowships from the University of Victoria and Bob Wright. Thanks to E Kim and G Prosperi-Porta, DFO, for maintaining and processing samples. Thanks to E Rondeau for amplicon sequencing and thanks to members of the Koop laboratory for support. Thanks to three anonymous reviewers for comments on an earlier version of the manuscript. en_US
dc.language.iso en en_US
dc.publisher Molecular Ecology en_US
dc.subject abiotic stress en_US
dc.subject copepod en_US
dc.subject ecological genomics en_US
dc.subject salinity en_US
dc.subject sea lice en_US
dc.subject transcriptomics en_US
dc.title Transcriptomics of coping strategies in free-swimming Lepeophtheirus salmonis (Copepoda) larvae responding to abiotic stress en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US

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