Transcriptome Analysis Provides Insight into Venom Evolution in a Seed-Parasitic Wasp, Megastigmus spermotrophus

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dc.contributor.author Paulson, Amber
dc.contributor.author Le, Cuong
dc.contributor.author Dickson, Jamie
dc.contributor.author Ehlting, Jürgen
dc.contributor.author von Aderkas, Patrick
dc.contributor.author Perlman, Steve
dc.date.accessioned 2017-07-31T17:44:49Z
dc.date.available 2017-07-31T17:44:49Z
dc.date.copyright 2016 en_US
dc.date.issued 2016-10
dc.identifier.citation Paulson, A.R., Le, C.H., Dickson, J.C., Ehlting, J., von Aderkas, P. & Perlman, S. J. (2016). Transcriptome analysis provides insight into venom evolution in a seedparasitic wasp, Megastigmus spermotrophus. Insect Molecular Biology, 25(5), 604- 616. https://doi.org/10.1111/imb.12247 en_US
dc.identifier.uri https://doi.org/10.1111/imb.12247
dc.identifier.uri https://dspace.library.uvic.ca//handle/1828/8388
dc.description.abstract One of the most striking host range transitions is the evolution of plant parasitism from animal parasitism. Parasitoid wasps that have secondarily evolved to attack plants (ie gall wasps and seed-feeders) demonstrate intimate associations with their hosts, yet the mechanism of plant–host manipulation is currently not known. There is, however, emerging evidence suggesting that ovipositional secretions play a role in plant manipulation. To investigate whether parasites have modified pre-existing adaptations to facilitate dramatic host shifts we aimed to characterize the expression of venom proteins in a plant parasite using a collection of parasitoid venom sequences as a guide. The transcriptome of a seed-feeding wasp, Megastigmus spermotrophus, was assembled de novo and three putative venoms were found to be highly expressed in adult females. One of these putative venoms, aspartylglucosaminidase, has been previously identified as a major venom component in two distantly related parasitoid wasps (Asobara tabida and Leptopilina heterotoma) and may have originated via gene duplication within the Hymenoptera. Our study shows that M. spermotrophus, a specialized plant parasite, expresses putative venom transcripts that share homology to venoms identified in Nasonia vitripennis (both superfamily Chalcidoidea), which suggests that M. spermotrophus may have co-opted pre-existing machinery to develop as a plant parasite. en_US
dc.description.sponsorship This work was funded by the Strategic Project Partnership Grants Program of NSERC and the Agence Nationale de Recherche - Programme Blanc 456 International. en_US
dc.language.iso en en_US
dc.publisher Insect Molecular Biology en_US
dc.subject venom en_US
dc.subject phytophagy en_US
dc.subject seed parasitism en_US
dc.subject Chalcidoidea en_US
dc.subject parasitoid en_US
dc.subject Hymenoptera en_US
dc.subject aspartylglucosaminidase en_US
dc.title Transcriptome Analysis Provides Insight into Venom Evolution in a Seed-Parasitic Wasp, Megastigmus spermotrophus en_US
dc.type Preprint en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US

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