Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
Date
2015
Authors
Percy, Andrew
Tamura-Wells, Jessica
Albar, Juan Pablo
Aloria, Kerman
Amirkhani, Ardeshir
Araujo, Gabriel D.T.
Arizmendi, Jesus M.
Blanco, Francisco J.
Canals, Francesc
Cho, Jin-Young
Journal Title
Journal ISSN
Volume Title
Publisher
EuPA Open Proteomics
Abstract
The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
Description
Keywords
Accuracy, Human plasma, Quality control, Quantitative proteomics, Reproducibility, Workflow validation
Citation
Percy, A.J., Tamura-Wells, J., Albar,J.P., Aloria, K., Amirkhani, A., Araujo, G. … Borchers, C.H. (2015). Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteomics, 8, 6-15. http://dx.doi.org/10.1016/j.euprot.2015.06.001