Identification and characterization of a novel LYR/LVR gene highly expressed during embryogenesis in Douglas-fir

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dc.contributor.author Ramachandran, Umesh
dc.date.accessioned 2010-02-22T17:36:02Z
dc.date.available 2010-02-22T17:36:02Z
dc.date.copyright 2006 en
dc.date.issued 2010-02-22T17:36:02Z
dc.identifier.uri http://hdl.handle.net/1828/2258
dc.description.abstract In order to elucidate the molecular and biochemical events occurring in embryogenesis in Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco), an essential gene expressed highly during early embryogenesis was identified, cloned and further characterized in this study. Douglas-fir LYR/LVR eDNA was obtained using RT-PCR with specific primers. followed by cloning and sequencing. Northern blot analysis showed higher amounts of LYR/LVR transcripts in early-cotyledonary embryonic stages and megagametophytes when compared with mid- and late-cotyledonary embryos. LYR/LVR transcript levels declined in seeds (mature embryos) and seedlings. Differential regulation of LYR/LVR gene expression with response to brassinosteroid treatment of Douglas-fir seeds was studied. LYR/LVR mRNA showed higher accumulation in seeds treated with different concentrations of brassinosteroids. Bioinformatic analysis showed that Douglas-fir LYR/LVR protein may be an essential inner mitochondrial protein, NADH oxidoreductase necessary for energy production. The phylogenetic tree analysis was used to investigate the evolutionary relationship of the newly identified Douglas-fir LYR/LVR protein with closely related proteins (LYR family) in different organisms. InterPro, UniProt and Pfam results showed the sequence similarity of Douglas-fir LYR/LVR protein with other related members in Arabidopsis thaliana and Oryza sativa, indicating that the LYR complex contains short stretches of closely related proteins that are essential for energy production. Amino acids 19-90 in the LYR/LVR protein were highly conserved and is likely the functional LYR motif necessary for oxidoreductase activity. en
dc.language English eng
dc.language.iso en en
dc.rights Available to the World Wide Web en
dc.subject Douglas fir en
dc.subject somatic embryogenesis en
dc.subject.lcsh UVic Subject Index::Sciences and Engineering::Biology::Microbiology en
dc.subject.lcsh UVic Subject Index::Sciences and Engineering::Chemistry::Biochemistry en
dc.title Identification and characterization of a novel LYR/LVR gene highly expressed during embryogenesis in Douglas-fir en
dc.type Thesis en
dc.contributor.supervisor Misra, Santosh
dc.degree.department Dept. of Biochemistry and Microbiology en
dc.degree.level Master of Science M.Sc. en

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