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Expression of BMAP18 in transgenic potato for production and enhanced disease resistance

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dc.contributor.author Francescutti, Teresa Marie
dc.date.accessioned 2010-03-01T17:52:04Z
dc.date.available 2010-03-01T17:52:04Z
dc.date.copyright 2007 en
dc.date.issued 2010-03-01T17:52:04Z
dc.identifier.uri http://hdl.handle.net/1828/2289
dc.description.abstract Cationic Antimicrobial Peptides (CAPs) exhibit broad-spectrum activity against a variety of microbial pathogens at concentrations that are non-toxic to higher eukaryotes. These properties make them excellent candidates for both pharmaceutical and agricultural applications. Here BMAP18, a CAP with an especially high charge to mass ratio, is evaluated for efficient production of the peptide and enhancement of disease resistance in transgenic potato. In vitro analyses indicated that BMAP18 had potent activity against a variety of clinically and agriculturally relevant pathogens, including the protozoan parasite Trypanosoma brucei. In planta activity was assessed by transformation of potato (Solanum tuberosum L.) plants with a synthetic BMAP18 gene under control of the enhanced CaMV 35S promoter or the Douglas-fir lumina] Binding Protein (PmBiP) promoter. Stable transformants expressing the transgene were shown to accumulate the peptide and exhibited enhanced resistance to Fusarium wilt and bacterial soft rot caused by Erwinia caratovora. en
dc.language English eng
dc.language.iso en en
dc.rights Available to the World Wide Web en
dc.subject plants en
dc.subject disease and pest resistance en
dc.subject peptide antibiotics en
dc.subject.lcsh UVic Subject Index::Sciences and Engineering::Chemistry::Biochemistry en
dc.subject.lcsh UVic Subject Index::Sciences and Engineering::Biology::Microbiology en
dc.title Expression of BMAP18 in transgenic potato for production and enhanced disease resistance en
dc.type Thesis en
dc.contributor.supervisor Misra, Santosh
dc.degree.department Dept. of Biochemistry and Microbiology en
dc.degree.level Master of Science M.Sc. en


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