An Affordable Microsphere-Based Device for Visual Assessment of Water Quality

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dc.contributor.author Rajwani, Azra
dc.contributor.author Restall, Brendon
dc.contributor.author Muller, Nathan J.
dc.contributor.author Roebuck, Scott
dc.contributor.author Willerth, Stephanie M.
dc.date.accessioned 2018-11-09T20:40:59Z
dc.date.available 2018-11-09T20:40:59Z
dc.date.copyright 2017 en_US
dc.date.issued 2017
dc.identifier.citation Rajwani, A., Restall, B., Muller, N., Roebuck, S. & Willerth, S. (2017) An Affordable Microsphere-Based Device for Visual Assessment of Water Quality. Biosensors, 7(3), 31. https://doi.org/10.3390/bios7030031 en_US
dc.identifier.uri https://doi.org/10.3390/bios7030031
dc.identifier.uri https://dspace.library.uvic.ca//handle/1828/10278
dc.description.abstract This work developed a prototype of an affordable, long-term water quality detection device that provides a visual readout upon detecting bacterial contamination. This device prototype consists of: (1) enzyme-releasing microspheres that lyse bacteria present in a sample, (2) microspheres that release probes that bind the DNA of the lysed bacteria, and (3) a detector region consisting of gold nanoparticles. The probes bind bacterial DNA, forming complexes. These complexes induce aggregation of the gold nanoparticles located in the detector region. The nanoparticle aggregation process causes a red to blue color change, providing a visual indicator of contamination being detected. Our group fabricated and characterized microspheres made of poly (ε-caprolactone) that released lysozyme (an enzyme that degrades bacterial cell walls) and hairpin DNA probes that bind to regions of the Escherichia coli genome over a 28-day time course. The released lysozyme retained its ability to lyse bacteria. We then showed that combining these components with gold nanoparticles followed by exposure to an E. coli-contaminated water sample (concentrations tested—106 and 108 cells/mL) resulted in a dramatic red to blue color change. Overall, this device represents a novel low-cost system for long term detection of bacteria in a water supply and other applications. en_US
dc.description.sponsorship Funding for this project was received from Grand Challenges Canada “Stars in Global Health” Program, the Canada Research Chairs Program and the British Columbia Innovation Council. We would also like to thank Alexandre Brolo and Milton Wang from the Department of Chemistry at the University of Victoria for providing us with the gold nanoparticles used in this study and for providing feedback on this manuscript. en_US
dc.language.iso en en_US
dc.publisher Biosensors en_US
dc.subject water quality en_US
dc.subject global health en_US
dc.subject colorimetric assay en_US
dc.subject drug delivery en_US
dc.subject microspheres en_US
dc.subject nanoparticles en_US
dc.title An Affordable Microsphere-Based Device for Visual Assessment of Water Quality en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US

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