Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization
Date
2017
Authors
Bosch-Presegué, Laia
Raurell-Vila, Helena
Thackray, Joshua K.
González, Jessica
Casal, Carmen
Kane-Goldsmith, Noriko
Vizoso, Miguel
Brown, Jeremy P.
Antonio, Gómez
Ausió, Juan
Journal Title
Journal ISSN
Volume Title
Publisher
Cell Reports
Abstract
HP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1 alpha, HP1 beta, and HP1 gamma play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1 alpha(-/-), Hp1 beta(-/-), and Hp1 gamma(-/-) MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1a confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1b is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability.
Description
Keywords
heterochromatin, HP1α, HP1β, HP1γ, Suv420h2, H4K20me3, genome organization, genome stability
Citation
Bosch-Presegué, L.; Raurell-Vila, H.; Thackray, J. K.; González, J.; Casal, C.; Kane-Goldsmith, N.; … & Vaquero, A. (2017). Mammalian HP1 isoforms have specific roles in heterochromatin structure and organization. Cell Reports, 21(8), 2048- 2057. DOI: 10.1016/j.celrep.2017.10.092