Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization

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dc.contributor.author Bosch-Presegué, Laia
dc.contributor.author Raurell-Vila, Helena
dc.contributor.author Thackray, Joshua K.
dc.contributor.author González, Jessica
dc.contributor.author Casal, Carmen
dc.contributor.author Kane-Goldsmith, Noriko
dc.contributor.author Vizoso, Miguel
dc.contributor.author Brown, Jeremy P.
dc.contributor.author Antonio, Gómez
dc.contributor.author Ausió, Juan
dc.contributor.author Zimmermann, Timo
dc.contributor.author Esteller, Manel
dc.contributor.author Schotta, Gunnar
dc.contributor.author Singh, Prim B.
dc.contributor.author Serrano, Lourdes
dc.contributor.author Vaquero, Alejandro
dc.date.accessioned 2019-03-21T00:14:02Z
dc.date.available 2019-03-21T00:14:02Z
dc.date.copyright 2017 en_US
dc.date.issued 2017
dc.identifier.citation Bosch-Presegué, L.; Raurell-Vila, H.; Thackray, J. K.; González, J.; Casal, C.; Kane-Goldsmith, N.; … & Vaquero, A. (2017). Mammalian HP1 isoforms have specific roles in heterochromatin structure and organization. Cell Reports, 21(8), 2048- 2057. DOI: 10.1016/j.celrep.2017.10.092 en_US
dc.identifier.uri https://doi.org/10.1016/j.celrep.2017.10.092
dc.identifier.uri http://hdl.handle.net/1828/10657
dc.description.abstract HP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1 alpha, HP1 beta, and HP1 gamma play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1 alpha(-/-), Hp1 beta(-/-), and Hp1 gamma(-/-) MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1a confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1b is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability. en_US
dc.description.sponsorship We thank the members of the Vaquero laboratory, Dr. Karen Schindler, Dr. Sonia Guil, and Dr. Javier Rodriguez-Ubreva, for support and fruitful discussion; Raquel Garcia and the CRG Microscopy Unit for support on FLIM-FRET experiments; and David Lleres, Angus I. Lamond, Victor V. Lobanenkov, Mien-Chie Hung, Alan Underhill, Peter Hemmerich, Chris Wilson, and Masayuki Sekimata for sharing reagents. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (SAF2011-25860 and SAF2014-55964R to A.V.) and cofunded by FEDER funds/European Regional Development Fund (ERDF)-a way to build Europe, the Catalan Government Agency AGAUR (2009SGR-914 and 2014SGR-400 to A.V.), HGINJ (to L.S.), Deutsche Forschungsgemeinschaft (SFB1064 to G.S.), and the Canadian Institutes of Health Research (CIHR) (MOP-97878 to J.A.). en_US
dc.language.iso en en_US
dc.publisher Cell Reports en_US
dc.subject heterochromatin en_US
dc.subject HP1α en_US
dc.subject HP1β en_US
dc.subject HP1γ en_US
dc.subject Suv420h2 en_US
dc.subject H4K20me3 en_US
dc.subject genome organization en_US
dc.subject genome stability en_US
dc.title Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US

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