Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization

Bosch-Presegué, Laia; Raurell-Vila, Helena; Thackray, Joshua K.; González, Jessica; Casal, Carmen; Kane-Goldsmith, Noriko; Vizoso, Miguel; Brown, Jeremy P.; Antonio, Gómez; Ausió, Juan; Zimmermann, Timo; Esteller, Manel; Schotta, Gunnar; Singh, Prim B.; Serrano, Lourdes; Vaquero, Alejandro

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dc.contributor.author	Bosch-Presegué, Laia
dc.contributor.author	Raurell-Vila, Helena
dc.contributor.author	Thackray, Joshua K.
dc.contributor.author	González, Jessica
dc.contributor.author	Casal, Carmen
dc.contributor.author	Kane-Goldsmith, Noriko
dc.contributor.author	Vizoso, Miguel
dc.contributor.author	Brown, Jeremy P.
dc.contributor.author	Antonio, Gómez
dc.contributor.author	Ausió, Juan
dc.contributor.author	Zimmermann, Timo
dc.contributor.author	Esteller, Manel
dc.contributor.author	Schotta, Gunnar
dc.contributor.author	Singh, Prim B.
dc.contributor.author	Serrano, Lourdes
dc.contributor.author	Vaquero, Alejandro
dc.date.accessioned	2019-03-21T00:14:02Z
dc.date.available	2019-03-21T00:14:02Z
dc.date.copyright	2017	en_US
dc.date.issued	2017
dc.identifier.citation	Bosch-Presegué, L.; Raurell-Vila, H.; Thackray, J. K.; González, J.; Casal, C.; Kane-Goldsmith, N.; … & Vaquero, A. (2017). Mammalian HP1 isoforms have specific roles in heterochromatin structure and organization. Cell Reports, 21(8), 2048- 2057. DOI: 10.1016/j.celrep.2017.10.092	en_US
dc.identifier.uri	https://doi.org/10.1016/j.celrep.2017.10.092
dc.identifier.uri	http://hdl.handle.net/1828/10657
dc.description.abstract	HP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1 alpha, HP1 beta, and HP1 gamma play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1 alpha(-/-), Hp1 beta(-/-), and Hp1 gamma(-/-) MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1a confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1b is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability.	en_US
dc.description.sponsorship	We thank the members of the Vaquero laboratory, Dr. Karen Schindler, Dr. Sonia Guil, and Dr. Javier Rodriguez-Ubreva, for support and fruitful discussion; Raquel Garcia and the CRG Microscopy Unit for support on FLIM-FRET experiments; and David Lleres, Angus I. Lamond, Victor V. Lobanenkov, Mien-Chie Hung, Alan Underhill, Peter Hemmerich, Chris Wilson, and Masayuki Sekimata for sharing reagents. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (SAF2011-25860 and SAF2014-55964R to A.V.) and cofunded by FEDER funds/European Regional Development Fund (ERDF)-a way to build Europe, the Catalan Government Agency AGAUR (2009SGR-914 and 2014SGR-400 to A.V.), HGINJ (to L.S.), Deutsche Forschungsgemeinschaft (SFB1064 to G.S.), and the Canadian Institutes of Health Research (CIHR) (MOP-97878 to J.A.).	en_US
dc.language.iso	en	en_US
dc.publisher	Cell Reports	en_US
dc.subject	heterochromatin	en_US
dc.subject	HP1α	en_US
dc.subject	HP1β	en_US
dc.subject	HP1γ	en_US
dc.subject	Suv420h2	en_US
dc.subject	H4K20me3	en_US
dc.subject	genome organization	en_US
dc.subject	genome stability	en_US
dc.title	Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization	en_US
dc.type	Article	en_US
dc.description.scholarlevel	Faculty	en_US
dc.description.reviewstatus	Reviewed	en_US