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Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei

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dc.contributor.author Xiao, Yao
dc.contributor.author Yi, Fei
dc.contributor.author Ling, Juanjuan
dc.contributor.author Wang, Zhi
dc.contributor.author Zhao, Kun
dc.contributor.author Lu, Nan
dc.contributor.author Qu, Guanzheng
dc.contributor.author Kong, Lisheng
dc.contributor.author Ma, Wenjun
dc.contributor.author Wang, Junhui
dc.date.accessioned 2020-03-09T14:14:37Z
dc.date.available 2020-03-09T14:14:37Z
dc.date.copyright 2020 en_US
dc.date.issued 2020
dc.identifier.citation Xiao, Y., Yi, F., Ling, J., Wang, Z., Zhao, K., Lu, N., Qu, G., Kong, L., Ma, W. & Wang, J. (2020). Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei. International Journal of Molecular Sciences, 21(5), 1686. https://doi.org/10.3390/ijms21051686 en_US
dc.identifier.uri https://doi.org/10.3390/ijms21051686
dc.identifier.uri http://hdl.handle.net/1828/11610
dc.description.abstract Catalpa bungei is an economically important tree with high-quality wood and highly valuable to the study of wood formation. In this work, the xylem microstructure of C. bungei tension wood (TW) was observed, and we performed transcriptomics, proteomics and Raman spectroscopy of TW, opposite wood (OW) and normal wood (NW). The results showed that there was no obvious gelatinous layer (G-layer) in the TW of C. bungei and that the secondary wall deposition in the TW was reduced compared with that in the OW and NW. We found that most of the differentially expressed mRNAs and proteins were involved in carbohydrate polysaccharide synthesis. Raman spectroscopy results indicated that the cellulose and pectin content and pectin methylation in the TW were lower than those in the OW and NW, and many genes and proteins involved in the metabolic pathways of cellulose and pectin, such as galacturonosyltransferase (GAUT), polygalacturonase (PG), endoglucanase (CLE) and β-glucosidase (BGLU) genes, were significantly upregulated in TW. In addition, we found that the MYB2 transcription factor may regulate the pectin degradation genes PG1 and PG3, and ARF, ERF, SBP and MYB1 may be the key transcription factors regulating the synthesis and decomposition of cellulose. In contrast to previous studies on TW with a G-layer, our results revealed a change in metabolism in TW without a G-layer, and we inferred that the change in the pectin type, esterification and cellulose characteristics in the TW of C. bungei may contribute to high tensile stress. These results will enrich the understanding of the mechanism of TW formation. en_US
dc.description.sponsorship This work was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002) en_US
dc.language.iso en en_US
dc.publisher International Journal of Molecular Science en_US
dc.subject tension wood en_US
dc.subject transcriptome en_US
dc.subject proteomics en_US
dc.subject Raman spectroscopy en_US
dc.subject cellulose en_US
dc.subject pectin en_US
dc.subject Catalpa bungei en_US
dc.title Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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