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Microfluidic Integration of a Double-Nanohole Optical Trap with Applications

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dc.contributor.author Zehtabi-Oskuie, Ana
dc.date.accessioned 2013-09-05T20:09:50Z
dc.date.available 2013-09-05T20:09:50Z
dc.date.copyright 2013 en_US
dc.date.issued 2013-09-05
dc.identifier.uri http://hdl.handle.net/1828/4916
dc.description.abstract This thesis presents optical trapping of various single nanoparticles, and the method for integrating the optical trap system into a microfluidic channel to examine the trapping stiffness and to study binding at the single molecule level. Optical trapping is the capability to immobilize, move, and manipulate small objects in a gentle way. Conventional trapping methods are able to trap dielectric particles with size greater than 100 nm. Optical trapping using nanostructures has overcome this limitation so that it has been of interest to trap nanoparticles for bio-analytical studies. In particular, aperture optical trapping allows for trapping at low powers, and easy detection of the trapping events by noting abrupt jumps in the transmission intensity of the trapping beam through the aperture. Improved trapping efficiency has been achieved by changing the aperture shape from a circle; for example, to a rectangle, double nanohole (DNH), or coaxial aperture. The DNH has the advantage of a well-defined trapping region between the two cusps where the nanoholes overlap, which typically allows only single particle trapping due to steric hindrance. Trapping of 21 nm encapsulated quantum dot has been achieved which shows optical trapping can be used in technologies that seek to place a quantum dot at a specific location in a plasmonic or nanophotonic structure. The DNH has been used to trap and unfold a single protein. The high signal-to-noise ratio of 33 in monitoring single protein trapping and unfolding shows a tremendous potential for using the double nanohole as a sensor for protein binding events at a single molecule level. The DNH integrated in a microfluidic chip with flow to show that stable trapping can be achieved under reasonable flow rates of a few µL/min. With such stable trapping under flow, it is possible to envision co-trapping of proteins to study their interactions. Co-trapping is achieved for the case where we flow in a protein (bovine serum albumin – BSA) and co-trap its antibody (anti-BSA). en_US
dc.language English eng
dc.language.iso en en_US
dc.subject Optical trapping en_US
dc.subject Binding en_US
dc.subject co-trapping en_US
dc.subject Microfluidic en_US
dc.subject Aperture en_US
dc.subject Quantum dot en_US
dc.title Microfluidic Integration of a Double-Nanohole Optical Trap with Applications en_US
dc.type Thesis en_US
dc.contributor.supervisor Gordon, Reuven
dc.degree.department Dept. of Electrical and Computer Engineering en_US
dc.degree.level Master of Applied Science M.A.Sc. en_US
dc.rights.temp Available to the World Wide Web en_US
dc.identifier.bibliographicCitation ZEHTABI-OSKUIE, A., JIANG, H., CYR, B.R., RENNEHAN, D.W., AL-BALUSHI, A.A., GORDON, R., “DOUBLE NANOHOLE OPTICAL TRAPPING: DYNAMICS AND PROTEIN-ANTIBODY CO-TRAPPING”, LAB ON A CHIP, 2013. en_US
dc.identifier.bibliographicCitation ZEHTABI-OSKUIE, A., BERGERON, J.G., GORDON, R., “FLOW-DEPENDENT DOUBLE-NANOHOLE OPTICAL TRAPPING OF 20 NM POLYSTYRENE NANOSPHERES”, SCI REP., 2012. en_US
dc.identifier.bibliographicCitation BERGERON, J.G., ZEHTABI-OSKUIE, A., GHAFFARI, S., PANG, Y., GORDON, R., “OPTICAL TRAPPING OF NANOPARTICLES”, JOVE, 2013. en_US
dc.identifier.bibliographicCitation ZEHTABI-OSKUIE, A., BERGERON, J.G., PANG, Y., MOFFITT, M., GORDON, R., “OPTICAL TRAPPING OF AN ENCAPSULATED QUANTUM DOT USING A DOUBLE NANOHOLE APERTURE IN A METAL FILM”, SPIE, 2012. en_US
dc.identifier.bibliographicCitation ZEHTABI-OSKUIE, A., GORDON, R., “NANOAPERTURE OPTICAL CO-TRAPPING OF A PROTEIN-ANTIBODY PAIR”, OSA, 2013. en_US
dc.description.scholarlevel Graduate en_US
dc.description.proquestcode 0544 en_US
dc.description.proquestcode 0752 en_US


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