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Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates

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dc.contributor.author Dijkstra, Johannes M
dc.contributor.author Grimholt, Unni
dc.contributor.author Leong, Jong
dc.contributor.author Koop, Ben F
dc.contributor.author Hashimoto, Keiichiro
dc.date.accessioned 2015-03-04T22:00:28Z
dc.date.available 2015-03-04T22:00:28Z
dc.date.copyright 2013 en_US
dc.date.issued 2013-11-26
dc.identifier.citation Dijkstra et al.: Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptideloading DM system in a large part of vertebrates. BMC Evolutionary Biology 2013 13:260. en_US
dc.identifier.uri http://www.biomedcentral.com/1471-2148/13/260
dc.identifier.uri http://dx.doi.org/10.1186/1471-2148-13-260
dc.identifier.uri http://hdl.handle.net/1828/5895
dc.description BioMed Central en_US
dc.description.abstract Background: Classical major histocompatibility complex (MHC) class II molecules play an essential role in presenting peptide antigens to CD4+ T lymphocytes in the acquired immune system. The non-classical class II DM molecule, HLA-DM in the case of humans, possesses critical function in assisting the classical MHC class II molecules for proper peptide loading and is highly conserved in tetrapod species. Although the absence of DM-like genes in teleost fish has been speculated based on the results of homology searches, it has not been definitively clear whether the DM system is truly specific for tetrapods or not. To obtain a clear answer, we comprehensively searched class II genes in representative teleost fish genomes and analyzed those genes regarding the critical functional features required for the DM system. Results: We discovered a novel ancient class II group (DE) in teleost fish and classified teleost fish class II genes into three major groups (DA, DB and DE). Based on several criteria, we investigated the classical/non-classical nature of various class II genes and showed that only one of three groups (DA) exhibits classical-type characteristics. Analyses of predicted class II molecules revealed that the critical tryptophan residue required for a classical class II molecule in the DM system could be found only in some non-classical but not in classical-type class II molecules of teleost fish. Conclusions: Teleost fish, a major group of vertebrates, do not possess the DM system for the classical class II peptide-loading and this sophisticated system has specially evolved in the tetrapod lineage. en_US
dc.description.sponsorship This work was supported by JSPS KAKENHI Grant Number 22580213 and 25450310 (to J. M. D.), a Norwegian Research Council grant (Havbruk # NFR 185217/S40) (to U. G.), an NSERC grant (to B. F. K.) and the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2008–2012. en_US
dc.language.iso en en_US
dc.publisher BMC Evolutionary Biology en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject MHC en_US
dc.subject Class II en_US
dc.subject Classical en_US
dc.subject Non-classical en_US
dc.subject DM en_US
dc.subject Peptide-loading en_US
dc.subject Teleost fish en_US
dc.subject Genomics en_US
dc.subject Evolution en_US
dc.title Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates en_US
dc.type Article en_US
dc.rights.temp Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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