UVicSpace

The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds

Show simple item record

dc.contributor.author Willerth, Stephanie
dc.contributor.author Rader, Allison
dc.contributor.author Sakiyama-Elbert, Shelly E
dc.date.accessioned 2015-07-03T21:24:13Z
dc.date.available 2015-07-03T21:24:13Z
dc.date.copyright 2008 en_US
dc.date.issued 2008-06-10
dc.identifier.citation Willerth, SM et al. The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds Stem Cell Res 1(3): 205-218 en_US
dc.identifier.uri http://www.sciencedirect.com/science/article/pii/S187350610800038X
dc.identifier.uri http://dx.doi.org/10.1016/j.scr.2008.05.006
dc.identifier.uri http://hdl.handle.net/1828/6287
dc.description.abstract The goal of this project is to develop 3-D biomaterial scaffolds that present cues to direct differentiation of embryonic stem cell derived neural progenitor cells (ESNPCs) seeded inside into mature neural phenotypes, specifically neurons and oligodendrocytes. Release studies were performed to determine the appropriate conditions for retention of neurotrophin-3 (NT-3), sonic hedgehog (Shh), and platelet derived growth factor (PDGF) by an affinity-based delivery system (ABDS) incorporated into fibrin scaffolds. Embryoid bodies (EBs) containing neural progenitors were formed from mouse ES cells, using a 4 − /4+ retinoic acid treatment protocol, and then seeded inside of fibrin scaffolds containing the drug delivery system. This delivery system was used to deliver various growth factor doses and combinations to the cells seeded inside of the scaffolds. Controlled delivery of NT-3 and PDGF simultaneously increased the fraction of neural progenitors, neurons, and oligodendrocytes while decreasing the fraction of astrocytes obtained compared to control cultures seeded inside of unmodified fibrin scaffolds with no growth factors present in the media. These results demonstrate that such a strategy can be used to generate an engineered tissue for the potential treatment of spinal cord injury and could be extended to study of differentiation in other tissues. en_US
dc.description.sponsorship This work was supported by NIH R01 NS051454. The authors thank Amy Boyet, Angela Guzmán, Nicole Moore, and Matthew Wood for technical support. The authors thank Shengzhou Wu and the Hope Center for Neurological Disorders, which is sup- ported by an NIH Neuroscience Blueprint Interdisciplinary Center Core grant (P30 NS057105), for use of the real-time PCR machine. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject murine embryonic stem cell en_US
dc.subject 3D culture en_US
dc.subject hydrogel en_US
dc.subject neural tissue engineering en_US
dc.title The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UVicSpace


Browse

My Account

Statistics

Help