Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture

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dc.contributor.author Regmi, Raju
dc.contributor.author Al Balushi, Ahmed A.
dc.contributor.author Rigneault, Hervé
dc.contributor.author Gordon, Reuven
dc.contributor.author Wenger, Jérôme
dc.date.accessioned 2016-04-22T20:59:54Z
dc.date.available 2016-04-22T20:59:54Z
dc.date.copyright 2015 en_US
dc.date.issued 2015-10-29
dc.identifier.citation Regmi, R., Al Balushi, A.A., Rigneault, H., Gordon, R. & Wenger, J. (2015). Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture. Nature: Scientific Reports, 5(15852), 1-8. en_US
dc.identifier.uri http://dx.doi.org/10.1038/srep15852
dc.identifier.uri http://hdl.handle.net/1828/7168
dc.description.abstract Diffraction ultimately limits the fluorescence collected from a single molecule, and sets an upper limit to the maximum concentration to isolate a single molecule in the detection volume. To overcome these limitations, we introduce here the use of a double nanohole structure with 25 nm gap, and report enhanced detection of single fluorescent molecules in concentrated solutions exceeding 20 micromolar. The nanometer gap concentrates the light into an apex volume down to 70 zeptoliter (10−21 L), 7000-fold below the diffraction-limited confocal volume. Using fluorescence correlation spectroscopy and time-correlated photon counting, we measure fluorescence enhancement up to 100-fold, together with local density of optical states (LDOS) enhancement of 30-fold. The distinctive features of double nanoholes combining high local field enhancement, efficient background screening and relative nanofabrication simplicity offer new strategies for real time investigation of biochemical events with single molecule resolution at high concentrations. en_US
dc.description.sponsorship The research leading to these results has received funding from the European Commission’s Seventh Framework Programme (FP7-ICT-2011-7) under grant agreements 288263 (NanoVista) and ERC StG 278242 (ExtendFRET) and from the NSERC Discovery Grant. RR is supported by the Erasmus Mundus Doctorate Program Europhotonics (Grant 159224-1-2009-1-FR-ERA MUNDUS-EMJD). en_US
dc.language.iso en en_US
dc.publisher Nature: Scientific Reports en_US
dc.rights Attribution 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ca/ *
dc.title Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US

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