Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro

Date

2023

Authors

Goncheva, Mariya I.
Heinrichs, David E.

Journal Title

Journal ISSN

Volume Title

Publisher

STAR Protocols

Abstract

Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with the optional extraction of host RNA and proteins. This protocol is applicable to many viral and bacterial strains and can be performed in different cell types.

Description

Keywords

Cell Culture, Microbiology, Molecular Biology

Citation

Goncheva, M. I., & Heinrichs, D. E. (2023). Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro. STAR Protocols, 4(3), 102411. https://doi.org/10.1016/j.xpro.2023.102411