Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture

dc.contributor.authorRegmi, Raju
dc.contributor.authorAl Balushi, Ahmed A.
dc.contributor.authorRigneault, Hervé
dc.contributor.authorGordon, Reuven
dc.contributor.authorWenger, Jérôme
dc.date.accessioned2016-04-22T20:59:54Z
dc.date.available2016-04-22T20:59:54Z
dc.date.copyright2015en_US
dc.date.issued2015-10-29
dc.description.abstractDiffraction ultimately limits the fluorescence collected from a single molecule, and sets an upper limit to the maximum concentration to isolate a single molecule in the detection volume. To overcome these limitations, we introduce here the use of a double nanohole structure with 25 nm gap, and report enhanced detection of single fluorescent molecules in concentrated solutions exceeding 20 micromolar. The nanometer gap concentrates the light into an apex volume down to 70 zeptoliter (10−21 L), 7000-fold below the diffraction-limited confocal volume. Using fluorescence correlation spectroscopy and time-correlated photon counting, we measure fluorescence enhancement up to 100-fold, together with local density of optical states (LDOS) enhancement of 30-fold. The distinctive features of double nanoholes combining high local field enhancement, efficient background screening and relative nanofabrication simplicity offer new strategies for real time investigation of biochemical events with single molecule resolution at high concentrations.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe research leading to these results has received funding from the European Commission’s Seventh Framework Programme (FP7-ICT-2011-7) under grant agreements 288263 (NanoVista) and ERC StG 278242 (ExtendFRET) and from the NSERC Discovery Grant. RR is supported by the Erasmus Mundus Doctorate Program Europhotonics (Grant 159224-1-2009-1-FR-ERA MUNDUS-EMJD).en_US
dc.identifier.citationRegmi, R., Al Balushi, A.A., Rigneault, H., Gordon, R. & Wenger, J. (2015). Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture. Nature: Scientific Reports, 5(15852), 1-8.en_US
dc.identifier.urihttp://dx.doi.org/10.1038/srep15852
dc.identifier.urihttp://hdl.handle.net/1828/7168
dc.language.isoenen_US
dc.publisherNature: Scientific Reportsen_US
dc.rightsAttribution 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/ca/*
dc.titleNanoscale volume confinement and fluorescence enhancement with double nanohole apertureen_US
dc.typeArticleen_US

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