Alford, Spencer C.O’Sullivan, ConnorObst, JonChristie, JenniferHoward, Perry L.2020-10-092020-10-0920142014Alford, S. C., Christie, J., Howard, P. L., Obst, J., & O’Sullivan, C. (2014). Conditional protein splicing of a-sarcin in live cells. Molecular BioSystems, 10(4), 831-837. https://doi.org/10.1039/c3mb70387hhttps://doi.org/10.1039/c3mb70387hhttp://hdl.handle.net/1828/12193Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.enCentre for Biomedical ResearchArticleDepartment of Biochemistry and MicrobiologyDepartment of Biology