Gallo, CaraThomas, Suma S.Selinger, Allison J.Hof, FraserBohne, Cornelia2023-01-132023-01-1320212021Gallo, C., Thomas, S. S., Selinger, A. J., Hof, F., & Bohne, C. (2021). Mechanism of a disassembly-driven sensing system studied by stopped-flow kinetics. The Journal of Organic Chemistry, 86(15), 10782-10787. https://doi.org/10.1021/acs.joc.1c00959https://doi.org/10.1021/acs.joc.1c00959http://hdl.handle.net/1828/14662We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 (DD1) assembly, and nicotine binding were determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications. Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of the system.enAnatomyFluorescenceKinetic parametersKineticsNicotineCentre for Advanced Materials and Related Technology (CAMTEC)ArticleDepartment of Chemistry