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Applications of Raman spectroscopy in radiation oncology: clinical instrumentation and radiation response signatures in tissue

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dc.contributor.author Van Nest, Samantha J
dc.date.accessioned 2018-08-31T18:58:41Z
dc.date.copyright 2018 en_US
dc.date.issued 2018-08-31
dc.identifier.uri https://dspace.library.uvic.ca//handle/1828/10006
dc.description.abstract Radiation therapy (RT) plays a crucial role in the management of cancer, however, current standards of care have yet to account for patient specific radiation sensitivity. Raman spectroscopy (RS) is a promising technique for radiobiological studies as a way to measure radiation responses in biological samples and could provide a method for monitoring and predicting radiation response in patients. The work in this dissertation gives way to significant advances in the implementation of RS for applications in radiation oncology. Specifically, instrumentation improvements for clinical implementation of RS were achieved through the investigation and development of Raman microfluidic systems. Unique magnesium fluoride based microfluidic systems were engineered and evaluated for applications in radiobiological studies. These systems were found to yield superior spectral quality over traditional microfluidic designs. Furthermore, in order to assert RS as a key technique for clinical monitoring and prediction of radiation responses, human non-small cell lung cancer (NSCLC) and breast adenocarcinoma tumour xenograft models were investigated for Raman signatures of radiation response. These studies found that RS can identify unique and distinct signatures of radiation response in tumours, that can be tracked over time. In particular, NSCLC tumours were found to have key radiation induced modulations in cell cycle and metabolic linked spectral features- including glycogen. Breast adenocarcinoma tumours were found to exhibit distinct fluctuations in spectral features linked to cell cycle as well as protein content. In the case of NSCLC, radiation response signatures were found to be linked to tumour regression and hypoxic status of the tumour- a key factor that dictates radiation resistance in the disease. This work provides the first application of RS to measure radiation response signatures of tumours irradiated \textit{in vivo}. These results show that RS is a versatile technique that can offer insight into radiation induced molecular changes that are unique to the type of cancer and can be monitored over several days following radiation exposure. Together with improved instrumentation for radiobiological studies using microfluidics, the work presented in this dissertation further emphasizes the key role RS can have in radiation oncology and personalization of RT. en_US
dc.language English eng
dc.language.iso en en_US
dc.rights Available to the World Wide Web en_US
dc.subject Raman spectroscopy en_US
dc.subject Radiation Oncology en_US
dc.subject Radiation biology en_US
dc.subject radiobiology en_US
dc.subject Medical Physics en_US
dc.subject Tumour Metabolism en_US
dc.subject non-small cell lung cancer en_US
dc.subject breast cancer en_US
dc.subject Immunofluorescence en_US
dc.title Applications of Raman spectroscopy in radiation oncology: clinical instrumentation and radiation response signatures in tissue en_US
dc.type Thesis en_US
dc.contributor.supervisor Jirasek, Andrew
dc.degree.department Department of Physics and Astronomy en_US
dc.degree.level Doctor of Philosophy Ph.D. en_US
dc.identifier.bibliographicCitation Van Nest, S. J., Nicholson, L. M., DeVorkin, L., Brolo, A. G., Lum, J. J., & Jirasek, A. (2018). Raman Spectroscopic Signatures Reveal Distinct Biochemical and Temporal Changes in Irradiated Human Breast Adenocarcinoma Xenografts. Radiation research, 189(5), 497-504. en_US
dc.identifier.bibliographicCitation Harder, S. J., Isabelle, M., DeVorkin, L., Smazynski, J., Beckham, W., Brolo, A. G., Lum, J.J., & Jirasek, A. (2016). Raman spectroscopy identifies radiation response in human non-small cell lung cancer xenografts. Scientific reports, 6, 21006. en_US
dc.description.scholarlevel Graduate en_US
dc.description.embargo 2019-08-21


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