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Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii

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dc.contributor.author Wang, Nan
dc.contributor.author Zhu, Tianqing
dc.contributor.author Lu, Nan
dc.contributor.author Wang, Zhi
dc.contributor.author Yang, Guijuan
dc.contributor.author Qu, Guanzheng
dc.contributor.author Kong, Lisheng
dc.contributor.author Zhang, Shougong
dc.contributor.author Ma, Wenjun
dc.contributor.author Wang, Junhui
dc.date.accessioned 2019-04-26T23:09:58Z
dc.date.available 2019-04-26T23:09:58Z
dc.date.copyright 2019 en_US
dc.date.issued 2019
dc.identifier.citation Wang, N., Zhu, T., Lu, N., Wang, Z., Guijuan, Y., Qu, G., … Wang, J. (2019). Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii. International Journal of Molecular Sciences, 20(8), 1895. https://doi.org/10.3390/ijms20081895 en_US
dc.identifier.uri http://dx.doi.org/10.3390/ijms20081895
dc.identifier.uri http://hdl.handle.net/1828/10762
dc.description.abstract Variegated plants are valuable materials for investigating leaf color regulated mechanisms. To unveil the role of posttranslational modification in the variegated phenotype, we conducted global quantitative phosphoproteomic analysis on different leaf color sectors of Maiyuanjinqiu and the corresponding of Catalpa fargesii using Ti4+-IMAC phosphopeptide enrichment. A total of 3778 phosphorylated sites assigned to 1646 phosphoproteins were identified, and 3221 in 1434 proteins were quantified. Differential phosphoproteins (above 1.5 or below 1/1.5) in various leaf color sectors were selected for functional enrichment analyses. Gene ontology (GO) enrichment revealed that processes of photosynthesis, regulation of the generation of precursor metabolites, response to stress, homeostasis, amino acid metabolism, transport–related processes, and most of the energy metabolisms might contribute to leaf color. KEGG pathway enrichment analysis was performed based on differential phosphoproteins (DPs) in different organelles. The result showed that most enriched pathways were located in the chloroplasts and cytosol. The phosphorylation levels of glycometabolism enzymes might greatly affect leaf variegation. Measurements of fluorescence parameters and enzyme activities confirmed that protein phosphorylation could affect plant physiology by regulating enzyme activity. These results provide new clues for further study the formation mechanisms of naturally variegated phenotype. en_US
dc.description.sponsorship This research was supported by Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002). en_US
dc.language.iso en en_US
dc.publisher International Journal of Molecular Sciences en_US
dc.subject Catalpa fargesii en_US
dc.subject phosphoproteomics en_US
dc.subject variegated leaf en_US
dc.subject regulated mechanism en_US
dc.title Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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