Modulation of the Microtubule Network for Optimization of Nanoparticle Dynamics for the Advancement of Cancer Nanomedicine

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dc.contributor.authorBannister, Aaron
dc.contributor.authorDissanayake, Dushanthi
dc.contributor.authorKowalewski, Antonia
dc.contributor.authorCicon, Leah
dc.contributor.authorBromma, Kyle
dc.contributor.authorChithrani, Devika
dc.date.accessioned2020-07-13T14:09:21Z
dc.date.available2020-07-13T14:09:21Z
dc.date.copyright2020en_US
dc.date.issued2020
dc.description.abstractNanoparticles (NPs) have shown promise in both radiotherapy and chemotherapy. NPs are mainly transported along cellular microtubules (MTs). Docetaxel (DTX) is a commonly used chemotherapeutic drug that can manipulate the cellular MT network to maximize its clinical benefit. However, the effect of DTX on NP behaviour has not yet been fully elucidated. We used gold NPs of diameters 15 and 50 nm at a concentration of 0.2 nM to investigate the size dependence of NP behaviour. Meanwhile, DTX concentrations of 0, 10 and 50 nM were used to uphold clinical relevance. Our study reveals that a concentration of 50 nM DTX increased NP uptake by ~50% and their retention by ~90% compared to cells treated with 0 and 10 nM DTX. Smaller NPs had a 20-fold higher uptake in cells treated with 50 nM DTX vs. 0 and 10 nM DTX. With the treatment of 50 nm DTX, the cells became more spherical in shape, and NPs were redistributed closer to the nucleus. A significant increase in NP uptake and retention along with their intracellular distribution closer to the nucleus with 50 nM DTX could be exploited to target a higher dose to the most important target, the nucleus in both radiotherapy and chemotherapy.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research was funded by Natural Sciences and Engineering Research Council of Canada (NSERC), grant number RGPN-2017-04501 and The KB and LC were funded by NSERC.en_US
dc.identifier.citationBannister, A., Dissanayake, D., Kowalewski, A., Cicon, L., Bromma, K., & Chithrani, D. B. (2020). Modulation of the microtubule network for optimization of nanoparticle dynamics for the advancement of cancer nanomedicine. Bioengineering, 7(2). https://doi.org/10.3390/bioengineering7020056en_US
dc.identifier.urihttps://doi.org/10.3390/bioengineering7020056
dc.identifier.urihttp://hdl.handle.net/1828/11926
dc.language.isoenen_US
dc.publisherBioengineeringen_US
dc.subjectnanoparticlesen_US
dc.subjectdocetaxelen_US
dc.subjectmicrotubulesen_US
dc.subjectnanomedicineen_US
dc.subjectcanceren_US
dc.titleModulation of the Microtubule Network for Optimization of Nanoparticle Dynamics for the Advancement of Cancer Nanomedicineen_US
dc.typeArticleen_US

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