Protamines from liverwort are produced by post-translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones
dc.contributor.author | D'Ippolito, Robert Anthony | |
dc.contributor.author | Minamino, Naoki | |
dc.contributor.author | Rivera-Casas, Ciro | |
dc.contributor.author | Cheema, Manjinder S. | |
dc.contributor.author | Bai, Dina L. | |
dc.contributor.author | Kasinsky, Harold E. | |
dc.contributor.author | Shabanowitz, Jeffrey | |
dc.contributor.author | Eirin-Lopez, Jose M. | |
dc.contributor.author | Ueda, Takashi | |
dc.contributor.author | Hunt, Donald F. | |
dc.contributor.author | Ausio, Juan | |
dc.date.accessioned | 2021-01-28T23:15:44Z | |
dc.date.available | 2021-01-28T23:15:44Z | |
dc.date.copyright | 2019 | en_US |
dc.date.issued | 2019 | |
dc.description.abstract | Protamines are small, highly-specialized, arginine-rich, and intrinsically-disordered chromosomal proteins that replace histones during spermiogenesis in many organisms. Previous evidence supports the notion that, in the animal kingdom, these proteins have evolved from a primitive replication-independent histone H1 involved in terminal cell differentiation. Nevertheless, a direct connection between the two families of chromatin proteins is missing. Here, we primarily used electron transfer dissociation MS-based analyses, revealing that the protamines in the sperm of the liverwort Marchantia polymorpha result from post-translational cleavage of three precursor H1 histones. Moreover, we show that the mature protamines are further post-translationally modified by di-aminopropanelation, and previous studies have reported that they condense spermatid chromatin through a process consisting of liquid-phase assembly likely involving spinodal decomposition. Taken together, our results reveal that the interesting evolutionary ancestry of protamines begins with histone H1 in both the animal and plant kingdoms. | en_US |
dc.description.reviewstatus | Reviewed | en_US |
dc.description.scholarlevel | Faculty | en_US |
dc.identifier.citation | D’Ippolito, R. A., Minamino, N., Rivera-Casas, C., Cheema, M. S., Bai, D. L., Kasinsky, H. E., … Ausió, J. (2019). Protamines from liverwort are produced by post -translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones. Journal of Biological Chemistry, 294(44), 16364-16373. https://doi.org/10.1074/jbc.RA119.010316. | en_US |
dc.identifier.uri | https://doi.org/10.1074/jbc.RA119.010316 | |
dc.identifier.uri | http://hdl.handle.net/1828/12613 | |
dc.language.iso | en | en_US |
dc.publisher | Journal of Biological Chemistry | en_US |
dc.subject | 14-3-3 protein | en_US |
dc.subject | mass spectrometry (MS) | en_US |
dc.subject | histone | en_US |
dc.subject | electron microscopy (EM) | en_US |
dc.subject | chromatography | en_US |
dc.subject | di-aminopropanelation | en_US |
dc.subject | histone H1 | en_US |
dc.subject | Marchantia | en_US |
dc.subject | mass spectrometry | en_US |
dc.subject | protamines | en_US |
dc.title | Protamines from liverwort are produced by post-translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones | en_US |
dc.type | Article | en_US |
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