Protamines from liverwort are produced by post-translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones

dc.contributor.authorD'Ippolito, Robert Anthony
dc.contributor.authorMinamino, Naoki
dc.contributor.authorRivera-Casas, Ciro
dc.contributor.authorCheema, Manjinder S.
dc.contributor.authorBai, Dina L.
dc.contributor.authorKasinsky, Harold E.
dc.contributor.authorShabanowitz, Jeffrey
dc.contributor.authorEirin-Lopez, Jose M.
dc.contributor.authorUeda, Takashi
dc.contributor.authorHunt, Donald F.
dc.contributor.authorAusio, Juan
dc.date.accessioned2021-01-28T23:15:44Z
dc.date.available2021-01-28T23:15:44Z
dc.date.copyright2019en_US
dc.date.issued2019
dc.description.abstractProtamines are small, highly-specialized, arginine-rich, and intrinsically-disordered chromosomal proteins that replace histones during spermiogenesis in many organisms. Previous evidence supports the notion that, in the animal kingdom, these proteins have evolved from a primitive replication-independent histone H1 involved in terminal cell differentiation. Nevertheless, a direct connection between the two families of chromatin proteins is missing. Here, we primarily used electron transfer dissociation MS-based analyses, revealing that the protamines in the sperm of the liverwort Marchantia polymorpha result from post-translational cleavage of three precursor H1 histones. Moreover, we show that the mature protamines are further post-translationally modified by di-aminopropanelation, and previous studies have reported that they condense spermatid chromatin through a process consisting of liquid-phase assembly likely involving spinodal decomposition. Taken together, our results reveal that the interesting evolutionary ancestry of protamines begins with histone H1 in both the animal and plant kingdoms.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.identifier.citationD’Ippolito, R. A., Minamino, N., Rivera-Casas, C., Cheema, M. S., Bai, D. L., Kasinsky, H. E., … Ausió, J. (2019). Protamines from liverwort are produced by post -translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones. Journal of Biological Chemistry, 294(44), 16364-16373. https://doi.org/10.1074/jbc.RA119.010316.en_US
dc.identifier.urihttps://doi.org/10.1074/jbc.RA119.010316
dc.identifier.urihttp://hdl.handle.net/1828/12613
dc.language.isoenen_US
dc.publisherJournal of Biological Chemistryen_US
dc.subject14-3-3 proteinen_US
dc.subjectmass spectrometry (MS)en_US
dc.subjecthistoneen_US
dc.subjectelectron microscopy (EM)en_US
dc.subjectchromatographyen_US
dc.subjectdi-aminopropanelationen_US
dc.subjecthistone H1en_US
dc.subjectMarchantiaen_US
dc.subjectmass spectrometryen_US
dc.subjectprotaminesen_US
dc.titleProtamines from liverwort are produced by post-translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histonesen_US
dc.typeArticleen_US

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