Transcriptomic analysis of Douglas-fir megagametophyte development and abortion

Simple item page
dc.contributor.authorBoyes, Ian
dc.contributor.supervisorAderkas, P. von
dc.contributor.supervisorEhlting, Jurgen
dc.date.accessioned2013-08-30T21:16:40Z
dc.date.available2013-08-30T21:16:40Z
dc.date.copyright2013en_US
dc.date.issued2013-08-30
dc.degree.departmentDepartment of Biology
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractDouglas-fir develops a megagametophyte regardless of the pollination state of the ovule, whereas many other conifers develop a megagametophye in response to polli- nation. Megagametophytes in unfertilized ovules degrade two weeks following fertil- ization of the surrounding population. This is mediated by programmed cell death (PCD). Pollinated and unpollinated megagametophytes were dissected from Douglas- fir cones and extracted for RNA, which was then used as input for sequencing. A transcriptome was assembled from this data and expression levels were calculated. The data were fitted to quadratic regressions to produce coexpression groups. There is no clear upregulation of PCD effectors in the unpollinated megagametophyte. Po- tential regulators of megagametophyte fate are present in the data. Some are as- sociated with ABA signalling and proanthocyanadin biosynthesis while others share similarity to known regulators of PCD. Seed development processes are represented in the expression data, which support current knowledge of conifer seed development and provide targets for research.en_US
dc.description.proquestcode0369en_US
dc.description.proquestcode0309en_US
dc.description.proquestcode0817en_US
dc.description.proquestemailigboyes@gmail.comen_US
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/4886
dc.language.isoenen_US
dc.rights.tempAvailable to the World Wide Weben_US
dc.subjectDouglas-firen_US
dc.subjectseeden_US
dc.subjectovuleen_US
dc.subjectmegagametophyteen_US
dc.subjectgymnospermen_US
dc.subjectconiferen_US
dc.subjectMegastigmusen_US
dc.subjectpollenen_US
dc.subjectpollinationen_US
dc.subjectabortionen_US
dc.subjectembryoen_US
dc.subjectABySSen_US
dc.subjectRSEMen_US
dc.subjectproanthyocyanidinen_US
dc.subjecttranscriptomeen_US
dc.titleTranscriptomic analysis of Douglas-fir megagametophyte development and abortionen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Boyes_Ian_MSc_2013.pdf
Size:
3.05 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Electronic Theses and Dissertations (ETD)
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.