Identification and characterization of a novel LYR/LVR gene highly expressed during embryogenesis in Douglas-fir

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dc.contributor.authorRamachandran, Umesh
dc.contributor.supervisorMisra, Santosh
dc.date.accessioned2010-02-22T17:36:02Z
dc.date.available2010-02-22T17:36:02Z
dc.date.copyright2006en
dc.date.issued2010-02-22T17:36:02Z
dc.degree.departmentDept. of Biochemistry and Microbiologyen
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractIn order to elucidate the molecular and biochemical events occurring in embryogenesis in Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco), an essential gene expressed highly during early embryogenesis was identified, cloned and further characterized in this study. Douglas-fir LYR/LVR eDNA was obtained using RT-PCR with specific primers. followed by cloning and sequencing. Northern blot analysis showed higher amounts of LYR/LVR transcripts in early-cotyledonary embryonic stages and megagametophytes when compared with mid- and late-cotyledonary embryos. LYR/LVR transcript levels declined in seeds (mature embryos) and seedlings. Differential regulation of LYR/LVR gene expression with response to brassinosteroid treatment of Douglas-fir seeds was studied. LYR/LVR mRNA showed higher accumulation in seeds treated with different concentrations of brassinosteroids. Bioinformatic analysis showed that Douglas-fir LYR/LVR protein may be an essential inner mitochondrial protein, NADH oxidoreductase necessary for energy production. The phylogenetic tree analysis was used to investigate the evolutionary relationship of the newly identified Douglas-fir LYR/LVR protein with closely related proteins (LYR family) in different organisms. InterPro, UniProt and Pfam results showed the sequence similarity of Douglas-fir LYR/LVR protein with other related members in Arabidopsis thaliana and Oryza sativa, indicating that the LYR complex contains short stretches of closely related proteins that are essential for energy production. Amino acids 19-90 in the LYR/LVR protein were highly conserved and is likely the functional LYR motif necessary for oxidoreductase activity.en
dc.identifier.urihttp://hdl.handle.net/1828/2258
dc.languageEnglisheng
dc.language.isoenen
dc.rightsAvailable to the World Wide Weben
dc.subjectDouglas firen
dc.subjectsomatic embryogenesisen
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Biology::Microbiologyen
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Chemistry::Biochemistryen
dc.titleIdentification and characterization of a novel LYR/LVR gene highly expressed during embryogenesis in Douglas-firen
dc.typeThesisen

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