Proteomic changes in cancer cell lines as a result of bacterial infection

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dc.contributor.authorRen, Bo
dc.contributor.authorWeke, Kenneth
dc.contributor.authorHardie, Darryl
dc.contributor.authorGoncheva, Mariya I.
dc.contributor.authorHupp, Ted
dc.contributor.authorAlfaro, Javier Antonio
dc.contributor.authorPětrošová, Helena
dc.contributor.authorGoodlett, David R.
dc.date.accessioned2026-05-07T17:31:18Z
dc.date.available2026-05-07T17:31:18Z
dc.date.issued2025
dc.description.abstractBacterial infections have been implicated in shaping the tumor microenvironment (TME), but their effects on cancer cell proteomes remain unexplored. In this study, we analyzed proteomic changes in melanoma (A375) and ovarian cancer (OVCAR3) cell line models following infection with Staphylococcus aureus strain USA300 or Salmonella enterica strain SL1344 using mass spectrometry-based label-free quantitative proteomics. Bacterial infection leads to widespread changes in host protein expression in the cancer cells, with levels of proteins involved in mitochondrial metabolism, RNA processing, and cellular stress response all increasing in relative abundance. In contrast, proteins involved in DNA repair, cytoskeletal structure, vesicle trafficking, and cell cycle regulation were consistently downregulated. The magnitude of the observed changes varied by the cancer cell type. Understanding these interactions may provide new directions for the role of bacteria in tumor progression and therapeutic resistance.
dc.description.reviewstatusReviewed
dc.description.scholarlevelFaculty
dc.description.sponsorshipWe would like to thank Dr. Lisa Reynolds (University of Victoria, BC, Canada) for providing the S. enterica SL1344 strain. D.R.G. and the work performed at the University of Victoria-Genome BC Proteomics Centre (UVic-PC) was supported by funding to the BC Proteomics Centre (BCPC) from Genome British Columbia, for operations and technology development (374PRO). M.I.G. was supported by an NSERC Discovery Grant RGPIN-2024-04587. J.A.A. was supported by funding from the European Union's Horizon 2020 research and innovation program under grant agreement no. 101017453.
dc.identifier.citationRen, B., Weke, K., Hardie, D., Goncheva, M. I., Hupp, T., Alfaro, J. A., P?trošová, H., & Goodlett, D. R. (2025). Proteomic changes in cancer cell lines as a result of bacterial infection. Proteomics, e70062. https://doi.org/10.1002/pmic.70062
dc.identifier.urihttps://doi.org/10.1002/pmic.70062
dc.identifier.urihttps://hdl.handle.net/1828/23839
dc.language.isoen
dc.publisherProteomics
dc.rightsCC BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectUVic Genome BC Proteomics Centre
dc.subjectSexual and Reproductive Health and Rights (SRHR) Aspiration Research Cluster
dc.subjectbacterial infection
dc.subjectcancer cells
dc.subjectproteomics
dc.subjecttumor microenvironment
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleProteomic changes in cancer cell lines as a result of bacterial infection
dc.typeArticle

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