Development of a cell cross flow system




Chung, Jessica

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Single cell analysis devices have become important tools to obtain unique information on cells to improve current medical techniques, such as tissue engineering, or diagnosis of cancer at an early stage. This thesis documents the development of a "cell cross flow system" (CFS), which aims to capture magnetically tagged (MT) cells from a heterogeneous population of cells, and array these cells in pre-determined locations using magnetic force. The CFS integrates a “magnetic single cell micro array” (MSCMA), and a gasket assembly to achieve this. Current single cell technology, relevant fluid and magnetic theory, CFS design process, finite element method (FEM) simulation, and cross flow experiments are detailed in this thesis. The CFS was successful in capturing MT Jurkat cells, and the experimental results were consistent with the FEM simulation analysis. It was found that the CFS was capable of capturing MT Jurkat cells up to a ratio of 1 to 103 (MT to non-magnetically tagged cells) using a cell concentration of 105 cells/mL. Although these results are promising, non-magnetically tagged Jurkat cells were found to adhere to the chip and could not be easily removed. Several recommendations were suggested for future iterations, including changing the gasket assembly design, optimizing the flow rate and cell concentration, and using smaller trap sizes for the MSCMA design.



Single cell analysis, Cell cross flow