Choline: A Potential Neuroprotective Agent for Microglia Recovery from Prenatal Ethanol Exposure

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dc.contributor.authorSahasrabudhe, Charuta
dc.date.accessioned2023-03-18T05:52:24Z
dc.date.available2023-03-18T05:52:24Z
dc.date.copyright2023en_US
dc.date.issued2023-03-17
dc.description.abstractFetal alcohol spectrum disorder (FASD) encompasses a range of cognitive, physical and neurobehavioral deficits arising from perinatal alcohol exposure. We investigated the effects of postnatal ethanol exposure (at a time period equivalent to 3rd trimester in humans) on microglia in the hippocampus of adolescent rats. Additionally, we explored whether choline could be used as a neuroprotective agent to attenuate ethanol-induced effects on microglia. 79 rat pups were randomly assigned to 4 treatment groups; EtOH + Choline, EtOH + Saline, Sham + Choline, or Sham + Saline. From PD4-9 pups underwent ethanol or sham exposures, and then received choline or saline injections from PD10-30. On PD36, the rats were anesthetized and intracardial perfusions were performed using paraformaldehyde to fix the tissue. The tissue was sectioned into 50 µm slices which underwent a three-day IBA-1 immunohistochemical staining procedure, specific to microglia detection. The slides were then imaged at 10X using an Olympus brightfield BX51 microscope. IBA-1 cell density measures were quantified via an automated macro. Preliminary results showed non-significant differences in microglia density between conditions and across hippocampal regions of interest. However, expansion of the sample size and the addition of a morphology analysis is underway and will allow us to better understand the interactions of ethanol and choline on microglia.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelUndergraduateen_US
dc.description.sponsorshipJamie Cassels Undergraduate Research Awards (JCURA)en_US
dc.identifier.urihttp://hdl.handle.net/1828/14877
dc.language.isoenen_US
dc.subjectprenatal ethanol exposure (PNEE)en_US
dc.subjectneuroinflammationen_US
dc.subjectearly interventionen_US
dc.subjectcholineen_US
dc.subjectmicrogliaen_US
dc.subjectfetal alcohol spectrum disorder (FASD)en_US
dc.titleCholine: A Potential Neuroprotective Agent for Microglia Recovery from Prenatal Ethanol Exposureen_US
dc.typePosteren_US

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