Investigating the Role of Staphylococcus ureus Proteins During Co-infection with Influenza A virus
| dc.contributor.author | Therrien, Elise | |
| dc.date.accessioned | 2025-05-06T22:39:49Z | |
| dc.date.available | 2025-05-06T22:39:49Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Co-infection with S. aureus and influenza A virus increases the mortality rate of viral disease, and during co-infection, viral titre is increased. This work aimed to uncover bacterial factors that contribute to the increase in viral particle production during co-infection. A screen of the bacterial genome revealed that pro-viral activity is mediated by the sae gene. The genetic basis of this activity was confirmed with genetic complementation. In addition to encoding a two-component system, sae also regulates many bacterial virulence factors.The sae regulon was screened for pro-viral effects, and the fnbA gene was required for pro-viral activity. Bacterial adhesion and invasion assays were performed to investigate the interactions between FnbA and virus-infected cells at 1 hour and 4 hours post-viral infection. We concluded that FnbA is not required for adhesion but is required for invasion of virus-infected cells at all points in the viral life cycle. | |
| dc.description.reviewstatus | Reviewed | |
| dc.description.scholarlevel | Undergraduate | |
| dc.description.sponsorship | Jamie Cassels Undergraduate Research Awards (JCURA) | |
| dc.identifier.uri | https://hdl.handle.net/1828/22155 | |
| dc.publisher | University of Victoria | |
| dc.subject | Staphylococcus aureus | |
| dc.subject | influenza | |
| dc.subject | co-infection | |
| dc.subject | virus | |
| dc.subject | bacteria | |
| dc.subject | microbiology | |
| dc.title | Investigating the Role of Staphylococcus ureus Proteins During Co-infection with Influenza A virus | |
| dc.type | Poster |