Isolation and identification of eight genetic loci from the intracellular pathogen 'Francisella novicida'

Simple item page
dc.contributor.authorGray, Catherine Jeanen_US
dc.date.accessioned2024-08-13T22:56:15Z
dc.date.available2024-08-13T22:56:15Z
dc.date.copyright2000en_US
dc.date.issued2000
dc.degree.departmentDepartment of Biochemistry and Microbiology
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractShuttle transposon mutagenesis of the intracellular bacterial pathogen F. novicida resulted in eight mutants which showed decreased growth in intraperitoneal murine macrophages as compared to the wildtype strain. The genetic loci flanking the transposon were isolated and characterized by DNA sequencing. Five of the eight mutant clones showed similarity to database entries at the deduced amino acid level. One of the mutants, designated CG62, was interrupted in a presumed gene encoding a protein identical to a possible virulence factor found in F. tularensis. This protein is expressed only when the bacterium is growing inside macrophages, and when it is exposed to hydrogen peroxide. The protein has been termed Fip, for Francisella intramacrophage protein. Four of the F. novicida mutants had interrupted genetic regions similar to genes for a stress response protein, a purine biosynthesis protein, alanine racemase and a protein of unknown function found in E. coli and V. cholerae, respectively. The other three mutant strains appear to have been interrupted in unknown genetic regions, which may encode novel proteins involved in virulence.
dc.format.extent106 pages
dc.identifier.urihttps://hdl.handle.net/1828/17956
dc.rightsAvailable to the World Wide Weben_US
dc.titleIsolation and identification of eight genetic loci from the intracellular pathogen 'Francisella novicida'en_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
GRAY_Catherine_Jean_MSC_2000_1106039.pdf
Size:
34.21 MB
Format:
Adobe Portable Document Format
Download

Collections

Electronic Theses and Dissertations (ETD)
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.