Evaluation and expression of indolicidins and polyphemusin variants in plants for broad-spectrum disease resistance

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dc.contributor.authorBhargava, Apurva
dc.contributor.supervisorMisra, Santosh
dc.date.accessioned2009-11-23T17:33:54Z
dc.date.available2009-11-23T17:33:54Z
dc.date.copyright2005en
dc.date.issued2005
dc.degree.departmentDept. of Biochemistry and Microbiologyen
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractThe cationic antimicrobial peptides, indolicidins and polyphemusins, have shown activity against animal pathogenic viruses. The aim of this work was to evaluate the efficacy of these peptides for engineering plants with a broad-spectrum disease resistance, including viral diseases. TTC, Evan's blue and phytotoxicity assays showed low cytotoxicity of indolicidin and polyphemusin variants (10R, 11R and PV5) on tobacco leaf discs, protoplasts and plantlets. In vitro assays using these variants showed a broad-spectrum activity against plant pathogenic bacteria, fungi and viruses. An assessment of in planta activity was performed by expressing these cationic peptides in transgenic tobacco (Nicotiana tabacum var Xanthi). The disease assays using detached leaves from transgenic tobacco plants showed a broad-spectrum disease resistance against bacteria, fungi and 'TMV. Further work is needed on optimization of expression of these peptides and their combinations for successful application of this approach under greenhouse and field conditions.en
dc.identifier.urihttp://hdl.handle.net/1828/1882
dc.languageEnglisheng
dc.language.isoenen
dc.rightsAvailable to the World Wide Weben
dc.subjectcationicen
dc.subjectindolicidinsen
dc.subjectpolyphemusinsen
dc.subject.lcshUVic Subject Index::Sciences and Engineeringen
dc.titleEvaluation and expression of indolicidins and polyphemusin variants in plants for broad-spectrum disease resistanceen
dc.typeThesisen

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