Development of new supramolecular tools for studying the Histone Code

dc.contributor.authorMinaker, Samuel Anthony
dc.contributor.supervisorHof, Fraser Alan
dc.date.accessioned2012-06-14T14:56:42Z
dc.date.copyright2012en_US
dc.date.issued2012-06-14
dc.degree.departmentDept. of Chemistryen_US
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractThe covalent modifications to the histone 2A, 2B, 3, and 4 N-terminal tails that affect gene expression have been deemed the “Histone Code.” Mis-regulation of these signalling pathways is of great interest as are important in human disease. A variety of peptides containing post-translationally modified histone 3 and 4 sequences were read using a supramolecular sensor array approach, where two or three sensors gave a unique response for each analyte when compared to others. These sequences were chosen to determine what type of modifications could be read (phosphorylation, acetylation, methylation) and if this type of array would be suitable for reading analytes on which antibodies—the leading technology—typically perform poorly. It was found that three sensors, which operate in neutral aqueous solution, were able to discriminate 16 different histone analytes. Additionally, it was shown that this array could report simultaneously on both concentration and the identities of histone analytes.en_US
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/4005
dc.languageEnglisheng
dc.language.isoenen_US
dc.rights.tempAvailable to the World Wide Weben_US
dc.subjectSupramolecularen_US
dc.subjectSensorsen_US
dc.subjectSensor Arrayen_US
dc.subjectCalixareneen_US
dc.subjectHistone Codeen_US
dc.subjectEpigeneticsen_US
dc.titleDevelopment of new supramolecular tools for studying the Histone Codeen_US
dc.typeThesisen_US

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