A handheld bioprinter for multi-material printing of complex constructs

dc.contributor.authorPagan, Erik
dc.contributor.authorStefanek, Evan
dc.contributor.authorSeyfoori, Amir
dc.contributor.authorRazzaghi, Mahmood
dc.contributor.authorChehri, Behnad
dc.contributor.authorMousavi, Ali
dc.contributor.authorArnaldi, Pietro
dc.contributor.authorAjji, Zineb
dc.contributor.authorDartora, Daniela Ravizzoni
dc.contributor.authorDabiri, Seyed Mohammad Hossein
dc.contributor.authorNuyt, Anne Monique
dc.contributor.authorKhademhosseini, Ali
dc.contributor.authorSavoji, Houman
dc.contributor.authorAkbari, Mohsen
dc.date.accessioned2023-11-30T17:13:09Z
dc.date.available2023-11-30T17:13:09Z
dc.date.copyright2023en_US
dc.date.issued2023
dc.description.abstractIn situ bioprinting—the process of depositing bioinks at a defected area, has recently emerged as a versatile technology for tissue repair and restoration via site-specific delivery of pro-healing constructs. The ability to print multiple materials in situ is an exciting approach that allows simultaneous or sequential dispensing of different materials and cells to achieve tissue biomimicry. Herein, we report a modular handheld bioprinter that deposits a variety of bioinks in situ with exquisite control over their physical and chemical properties. Combined stereolithography 3D printing and microfluidic technologies allowed us to develop a novel low-priced handheld bioprinter. The ergonomic design of the handheld bioprinter facilitate the shape-controlled biofabrication of multi-component fibers with different cross-sectional shapes and material compositions. Furthermore, the capabilities of the produced fibers in the local delivery of therapeutic agents was demonstrated by incorporating drug-loaded microcarriers, extending the application of the printed fibers to on-demand, temporal, and dosage-control drug delivery platforms. Also, the versatility of this platform to produce biosensors and wearable electronics was demonstrated via incorporating conductive materials and integrating pH-responsive dyes. The handheld printer's efficacy in generating cell-laden fibers with high cell viability for site-specific cell delivery was shown by producing single-component and multi-component cell-laden fibers. In particular, the multi-component fibers were able to model the invasion of cancer cells into the adjacent tissue.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipM A acknowledges the support from the Natural Sciences and Engineering Research Council of Canada (NSERC, RGPIN-2016-04024) and the Canadian Foundation for Innovation (35570) and the B C Knowledge Development Fund (BCKDF). H S acknowledges the support received from the Natural Sciences and Engineering Research Council of Canada (NSERC) (NSERC, RGPIN-2021-03960, DGECR-2021-00337)Fonds de Recherche du Québec Santé (FRQS) (Chercheurs-boursiers J1 (313837) and Establishment of Young Investigators (324277), and Montreal TransMedTech Institute (iTMT). M A and E P thank support received from the Canadian Institutes for Health Research (CIHR, 201610PJT). A M acknowledges the Merit Scholarship of the Faculty of Medicine of the University of Montreal. M A and A K acknowledge the support received from Terasaki Institute for Biomedical Innovation.en_US
dc.identifier.citationPagan, E., Stefanek, E., Seyfoori, A., Razzaghi, M., Chehri, B., Mousavi, A.,... Akbari, M. (2023). A handheld bioprinter for multi-material printing of complex constructs. Biofabrication, 15(3), 035012. https://doi.org/10.1088/1758-5090/acc42cen_US
dc.identifier.urihttps://doi.org/10.1088/1758-5090/acc42c
dc.identifier.urihttp://hdl.handle.net/1828/15655
dc.language.isoenen_US
dc.publisherBiofabricationen_US
dc.subjectbioprintingen_US
dc.subjectin situ printingen_US
dc.subjectbioinksen_US
dc.subjectdrug deliveryen_US
dc.subjectcell deliveryen_US
dc.titleA handheld bioprinter for multi-material printing of complex constructsen_US
dc.typeArticleen_US

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