Functional characterization of PtMYB115, a regulator of condensed tannin synthesis in poplar

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dc.contributor.authorFranklin, Amy Midori
dc.contributor.supervisorConstabel, Carsten Peter
dc.date.accessioned2013-12-06T18:53:52Z
dc.date.available2013-12-06T18:53:52Z
dc.date.copyright2013en_US
dc.date.issued2013-12-06
dc.degree.departmentDepartment of Biology
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractCondensed tannins are wide-spread polyphenols with diverse ecological functions, including defense against herbivores and microbial pathogens. In poplar, condensed tannin synthesis is induced by a variety of biotic and abiotic stresses. The objective of this study was to determine the function of the R2R3 MYB transcription factor MYB115 in the regulation of condensed tannin synthesis. MYB115 was shown to be induced by wounding along with tannin biosynthetic genes and shows sequence similarity to characterized regulators of tannin synthesis in grape and persimmon suggesting that it functions in the regulation of condensed tannin synthesis. To analyze the function of MYB115, transgenic plants overexpressing MYB115 were generated and showed enhanced accumulation of condensed tannins and higher expression of flavonoid biosynthetic genes involved in condensed tannin biosynthesis compared to wild-type control plants. In promoter activation assays, MYB115 activated the promoter of a tannin-specific biosynthetic enzyme, anthocyanidin reductase. This suggests that MYB115 acts as a regulator of condensed tannin synthesis. MYB115 overexpressors showed additional changes to phenolic metabolism, including changes in levels of phenolic glycosides and hydroxycinnamic acids. These results indicate an important role of MYB115 in the regulation of the condensed tannin pathway in poplar.en_US
dc.description.proquestcode0817en_US
dc.description.proquestcode0307en_US
dc.description.proquestcode0487en_US
dc.description.proquestemailfrankla@uvic.caen_US
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/5065
dc.language.isoenen_US
dc.rights.tempAvailable to the World Wide Weben_US
dc.subjectcondensed tanninsen_US
dc.subjectR2R3 MYB transcription factorsen_US
dc.subjectpoplaren_US
dc.titleFunctional characterization of PtMYB115, a regulator of condensed tannin synthesis in poplaren_US
dc.typeThesisen_US

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