Paramecium bursaria Chlorella Virus 1 Proteome Reveals Novel Architectural and Regulatory Features of a Giant Virus
| dc.contributor.author | Dunigan, David D. | |
| dc.contributor.author | Cerny, Ronald L. | |
| dc.contributor.author | Bauman, Andrew T. | |
| dc.contributor.author | Roach, Jared C. | |
| dc.contributor.author | Lane, Leslie C. | |
| dc.contributor.author | Agarkova, Irina V. | |
| dc.contributor.author | Wulser, Kurt | |
| dc.contributor.author | Yanai-Balser, Giane M. | |
| dc.contributor.author | Gurnon, James R. | |
| dc.contributor.author | Vitek, Jason C. | |
| dc.contributor.author | Kronschnabel, Bernard J. | |
| dc.contributor.author | Jeanniard, Adrien | |
| dc.contributor.author | Blanc, Guillaume | |
| dc.contributor.author | Upton, Chris | |
| dc.contributor.author | Duncan, Garry A. | |
| dc.contributor.author | McClung, O. William | |
| dc.contributor.author | Ma, Fangrui | |
| dc.contributor.author | Van Etten, James L. | |
| dc.date.accessioned | 2016-06-27T23:34:54Z | |
| dc.date.available | 2016-06-27T23:34:54Z | |
| dc.date.copyright | 2012 | en_US |
| dc.date.issued | 2012-08 | |
| dc.description.abstract | The 331-kbp chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) genome was resequenced and annotated to correct errors in the original 15-year-old sequence; 40 codons was considered the minimum protein size of an open reading frame. PBCV-1 has 416 predicted protein-encoding sequences and 11 tRNAs. A proteome analysis was also conducted on highly purified PBCV-1 virions using two mass spectrometry-based protocols. The mass spectrometry-derived data were compared to PBCV-1 and its host Chlorella variabilis NC64A predicted proteomes. Combined, these analyses revealed 148 unique virus-encoded proteins associated with the virion (about 35% of the coding capacity of the virus) and 1 host protein. Some of these proteins appear to be structural/architectural, whereas others have enzymatic, chromatin modification, and signal transduction functions. Most (106) of the proteins have no known function or homologs in the existing gene databases except as orthologs with proteins of other chloroviruses, phycodnaviruses, and nuclear-cytoplasmic large DNA viruses. The genes encoding these proteins are dispersed throughout the virus genome, and most are transcribed late or early-late in the infection cycle, which is consistent with virion morphogenesis. | en_US |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | The UNL mass spectrometry facility is supported in part by NIH Grant P20 RR15635 from the COBRE Program of the National Center for Research Resources, NCI Cancer Center Support Grant P30 CA36727, and the Nebraska Research Initiative. Additional support was from NIH COBRE Grant P20RR015635 (D.D.D.), NIH R0l GM32441 (J.L.V.E.), NSF-EPSCoR EPS-1004094 (J.L.V.E.), DOE DE-EE0003142 (J.L.V.E.), a Discovery grant from NSERC (Canada) (C.U.), and National Center for Research Resources 5P20RR016469 and NIGMS 8P20GM103427 (G.D.). We thank Jaehyoung Kim and Joe Nietfeldt at the UNL Core for Applied Genomics and Ecology for the PBCV-1 sequencing. | en_US |
| dc.identifier.citation | Dunigan, D.D., Cerny, R.L., Bauman, A.T., Roach, J.C., Lane, L.C., Agarkova, I.V. … Van Etten, J.L. (2012). Paramecium bursaria chlorella virus 1 proteome reveals novel architectural and regulatory features of a giant virus. Journal of Virology, 86(16), 8821-8834. | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1128/JVI.00907-12 | |
| dc.identifier.uri | http://hdl.handle.net/1828/7372 | |
| dc.language.iso | en | en_US |
| dc.publisher | Journal of Virology | en_US |
| dc.title | Paramecium bursaria Chlorella Virus 1 Proteome Reveals Novel Architectural and Regulatory Features of a Giant Virus | en_US |
| dc.type | Article | en_US |