A lipidomics-based method to eliminate negative urine culture in general population

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dc.contributor.authorNartey, Linda K.
dc.contributor.authorMikhael, Abanoub
dc.contributor.authorP?trošová, Helena
dc.contributor.authorYuen, Victor
dc.contributor.authorKibsey, Pamela
dc.contributor.authorPekcan, Mert
dc.contributor.authorErnst, Robert K.
dc.contributor.authorChen, Michael X.
dc.contributor.authorGoodlett, David R.
dc.date.accessioned2026-05-07T17:31:17Z
dc.date.available2026-05-07T17:31:17Z
dc.date.issued2024
dc.description.abstractUrinary tract infections (UTIs) pose a significant challenge to human health. Accurate and timely detection remains pivotal for effective intervention. Current urine culture techniques, while essential, often encounter challenges where urinalysis yields positive results, but subsequent culture testing produces a negative result. This highlights potential discrepancies between the two methods and emphasizes the need for improved correlation in urinary tract infection (UTI) detection. Employing advanced lipidomics techniques, we deployed the fast lipid analysis technique (FLAT) on a clinical cohort suspected of having UTIs. Lipid fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), directly from urine samples without ex vivo growth, correctly identified the common uropathogens within a 1 hour timeframe when compared to urine culture. FLAT analysis also identified urine samples without culturable pathogens (negative UTIs) with 99% microbial identification (ID) agreement, whereas urinalysis showed 37% ID agreement with the gold standard urine culture. In 402 urine samples suspected for UTI from outpatients, FLAT assay rapidly ruled out negative urines without the need for culture in 77% of all cases. The potential impact of this innovative lipidomic-based approach extends beyond conventional diagnostic limitations, offering new avenues for early detection and targeted management of urinary tract infections. This research marks a paradigm shift in urine culture methodology, paving the way for improved clinical outcomes and public health interventions.
dc.description.reviewstatusReviewed
dc.description.scholarlevelFaculty
dc.description.sponsorshipProfs. Goodlett and Ernst thank the National Institutes of Health for funding from R01AI147314. Dr. Chen thanks the Vancouver Island Health Authority and Victoria hospitals foundation for funding from the Catalyst grant program. Prof. Pekcan thanks the Scientific and Technological Research Council of Turkey (TUBITAK) and the International Postdoctoral Research Fellowship Program (award 2219).
dc.identifier.citationNartey, L. K., Mikhael, A., P?trošová, H., Yuen, V., Kibsey, P., Pekcan, M., Ernst, R. K., Chen, M. X., & Goodlett, D. R. (2024). A lipidomics-based method to eliminate negative urine culture in general population. Journal of Clinical Microbiology, 62(10), e0081924. https://doi.org/10.1128/jcm.00819-24
dc.identifier.urihttps://doi.org/10.1128/jcm.00819-24
dc.identifier.urihttps://hdl.handle.net/1828/23835
dc.language.isoen
dc.publisherJournal of Clinical Microbiology
dc.rightsCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectUVic Genome BC Proteomics Centre
dc.subjectSexual and Reproductive Health and Rights (SRHR) Aspiration Research Cluster
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.subject.departmentDivision of Medical Sciences
dc.titleA lipidomics-based method to eliminate negative urine culture in general population
dc.typeArticle

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