Spatial effects on electrospray ionization response

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dc.contributor.authorJanusson, Eric
dc.contributor.authorHesketh, Amelia
dc.contributor.authorBamford, Karlee
dc.contributor.authorHatlelid, Katie
dc.contributor.authorHiggins, Rehan
dc.contributor.authorMcIndoe, J. Scott
dc.date.accessioned2017-04-24T20:54:48Z
dc.date.available2017-04-24T20:54:48Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.description.abstractThe position of the spray-head, the solvent, and a variety of additional instrumental parameters were independently adjusted during electrospray ionization mass spectrometric (ESI-MS) analysis of an equimolar mixture of two different ions. These parameters were found to have drastic effects on the distribution of signal intensity from one ion to another, and therefore the resulting usefulness of acquired spectra. The analytes studied were bis(triphenylphosphine)iminium (PPN) chloride and tetramethylammonium (TMA) chloride, two chemically distinct ions. The use of these two ions in a test solution yielded information regarding ESI probe spatial effects for two very different analytes, while probing the issue of sampling efficiency. Each experimental parameter was individually adjusted prior to rastering the spray head across the operational plane in order to observe how adjustment to a particular parameter affects analyte signal in relation to the distance from the MS aperture. Following acquisition, the intensities of both ions were plotted as ion contour maps demonstrating the intensity change with respect to capillary position in relation to the mass spectrometer aperture. The sharp contrast in ion intensity, and even differential ion activity, with relatively minor instrument changes (such as temperature programming, gas flow rates and solvent choice) clearly demonstrates the importance of finding the “sweet spot” for the ESI spray head, especially when signal intensity and a quality analysis is key.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipJSM thanks NSERC for operational funding (Discovery and Discovery Accelerator Supplement), and CFI, BCKDF and the University of Victoria for instrumental support. Additional thank-you to supplementary funding from Imperial Oil (Collaborative Research and Development Grant).en_US
dc.identifier.citationJanusson, E., Hesketh, A., Bamford, K., Hatlelid, K., Higgins, R., & McIndoe, J. (2015). Spatial effects on electrospray ionization response. International Journal of Mass Spectrometry, 388, 1-8.en_US
dc.identifier.urihttp://doi.org/10.1016/j.ijms.2015.07.016
dc.identifier.urihttp://hdl.handle.net/1828/7976
dc.language.isoenen_US
dc.publisherInternational Journal of Mass Spectrometryen_US
dc.subjectElectrospray ionizationen_US
dc.subjectsurface activityen_US
dc.subjectspatial effectsen_US
dc.subjectrelative intensityen_US
dc.titleSpatial effects on electrospray ionization responseen_US
dc.typePostprinten_US

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