The use of UV resonance Raman spectroscopy in the analysis of ionizing radiation-induced damage in DNA

dc.contributor.authorShaw, Conor Patrick
dc.contributor.supervisorJirasek, Andrew
dc.date.accessioned2007-12-14T01:18:16Z
dc.date.available2007-12-14T01:18:16Z
dc.date.copyright2007en_US
dc.date.issued2007-12-14T01:18:16Z
dc.degree.departmentDept. of Physics and Astronomyen_US
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractRaman spectroscopy is a form of vibrational spectroscopy that is capable of probing biological samples at a molecular level. In this work it was used in the analysis of ionizing radiation-induced damage in DNA. Spectra of both simple, short-stranded DNA oligomers (SS-DNA) and the more complicated calf-thymus DNA (CT-DNA) were acquired before and after irradiation to a variety of doses from 0 to ~2000 Gy. In a technique known as ultraviolet resonance Raman spectroscopy (UVRRS), three UV wavelengths of 248, 257 and 264 nm were utilized in order to selectively enhance contributions from different molecular groups within the samples. Assignment of the spectral peaks was aided by the literature, as well as through analysis of UVRR spectra of short strands of the individual DNA bases obtained at each of the three incident UV wavelengths. Difference spectra between the irradiated and unirradiated samples were calculated and the samples exposed to ~2000 Gy showed significant radiation-induced features. Intensity increases of spectral peaks, observed primarily in the CT-DNA, indicated unstacking of the DNA bases and disruption of Watson-Crick hydrogen bonds, while intensity decreases of spectral peaks, observed only in the SS-DNA, indicated both base damage and the loss of structural integrity of the DNA molecule. The high molecular specificity of UVRRS allowed for precise identification of the specific bonds affected by the radiation, and the use of the varying incident wavelengths allowed for the observation of damage to moieties that would otherwise have been excluded. The use of UVRRS shows promise in the study of radiation-induced damage to DNA and would be well suited for extension to the study of more complicated biological systems.en_US
dc.identifier.urihttp://hdl.handle.net/1828/271
dc.languageEnglisheng
dc.language.isoenen_US
dc.rightsAvailable to the World Wide Weben_US
dc.subjectradiationen_US
dc.subjectDNAen_US
dc.subjectradiation damageen_US
dc.subjectDNA damageen_US
dc.subjectRamanen_US
dc.subjectspectroscopyen_US
dc.subjectresonanceen_US
dc.subjectultravioleten_US
dc.subjectbase stackingen_US
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Physics::Radiationen_US
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Biology::Molecular biologyen_US
dc.titleThe use of UV resonance Raman spectroscopy in the analysis of ionizing radiation-induced damage in DNAen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
thesis_cps.pdf
Size:
5.19 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.18 KB
Format:
Item-specific license agreed upon to submission
Description: