Expression of BMAP18 in transgenic potato for production and enhanced disease resistance

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dc.contributor.authorFrancescutti, Teresa Marie
dc.contributor.supervisorMisra, Santosh
dc.date.accessioned2010-03-01T17:52:04Z
dc.date.available2010-03-01T17:52:04Z
dc.date.copyright2007en
dc.date.issued2010-03-01T17:52:04Z
dc.degree.departmentDept. of Biochemistry and Microbiologyen
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractCationic Antimicrobial Peptides (CAPs) exhibit broad-spectrum activity against a variety of microbial pathogens at concentrations that are non-toxic to higher eukaryotes. These properties make them excellent candidates for both pharmaceutical and agricultural applications. Here BMAP18, a CAP with an especially high charge to mass ratio, is evaluated for efficient production of the peptide and enhancement of disease resistance in transgenic potato. In vitro analyses indicated that BMAP18 had potent activity against a variety of clinically and agriculturally relevant pathogens, including the protozoan parasite Trypanosoma brucei. In planta activity was assessed by transformation of potato (Solanum tuberosum L.) plants with a synthetic BMAP18 gene under control of the enhanced CaMV 35S promoter or the Douglas-fir lumina] Binding Protein (PmBiP) promoter. Stable transformants expressing the transgene were shown to accumulate the peptide and exhibited enhanced resistance to Fusarium wilt and bacterial soft rot caused by Erwinia caratovora.en
dc.identifier.urihttp://hdl.handle.net/1828/2289
dc.languageEnglisheng
dc.language.isoenen
dc.rightsAvailable to the World Wide Weben
dc.subjectplantsen
dc.subjectdisease and pest resistanceen
dc.subjectpeptide antibioticsen
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Chemistry::Biochemistryen
dc.subject.lcshUVic Subject Index::Sciences and Engineering::Biology::Microbiologyen
dc.titleExpression of BMAP18 in transgenic potato for production and enhanced disease resistanceen
dc.typeThesisen

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