Characterization of the sperm chromatin structure in Mytilus californianus and Spisula solidissima

Simple item page
dc.contributor.authorZhang, Fanen_US
dc.date.accessioned2024-08-15T20:20:13Z
dc.date.available2024-08-15T20:20:13Z
dc.date.copyright1996en_US
dc.date.issued1996
dc.degree.departmentDepartment of Biochemistry and Microbiology
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractThe aim of this thesis was to establish the chromatin structure and organization in the sperm of two bivalve mollusks: Mytilus californianus and Spisula solidissima. Nuclease digestion and crosslinking studies showed that the sperm chromatin of Mytilus califomianus has a organization consisting of small groups of oligonucleosomes interspersed between long stretches of nucleoprotamine filaments. The nucleoprotamine complexes result from the association of PL-II, PL-III and PL-IV with DNA. The spatial arrangement of different PL proteins within these complexes is discussed. In the case of Spisula, it was found that its major protamine-like component (PL-I) contains at least two cysteines located between tryptophan and tyrosine residues of the globular region of the molecule. It was also found that the PL-I exhibits a head to tail alignment in the sperm.
dc.format.extent74 pages
dc.identifier.urihttps://hdl.handle.net/1828/20265
dc.rightsAvailable to the World Wide Weben_US
dc.titleCharacterization of the sperm chromatin structure in Mytilus californianus and Spisula solidissimaen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Zhang_Fan_MSc_1991_694920.pdf
Size:
29.17 MB
Format:
Adobe Portable Document Format
Download

Collections

Electronic Theses and Dissertations (ETD)
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.