Characterization of the sperm chromatin structure in Mytilus californianus and Spisula solidissima

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dc.contributor.authorZhang, Fanen_US
dc.date.accessioned2024-08-15T20:20:13Z
dc.date.available2024-08-15T20:20:13Z
dc.date.copyright1996en_US
dc.date.issued1996
dc.degree.departmentDepartment of Biochemistry and Microbiologyen_US
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractThe aim of this thesis was to establish the chromatin structure and organization in the sperm of two bivalve mollusks: Mytilus californianus and Spisula solidissima. Nuclease digestion and crosslinking studies showed that the sperm chromatin of Mytilus califomianus has a organization consisting of small groups of oligonucleosomes interspersed between long stretches of nucleoprotamine filaments. The nucleoprotamine complexes result from the association of PL-II, PL-III and PL-IV with DNA. The spatial arrangement of different PL proteins within these complexes is discussed. In the case of Spisula, it was found that its major protamine-like component (PL-I) contains at least two cysteines located between tryptophan and tyrosine residues of the globular region of the molecule. It was also found that the PL-I exhibits a head to tail alignment in the sperm.
dc.format.extent74 pages
dc.identifier.urihttps://hdl.handle.net/1828/20265
dc.rightsAvailable to the World Wide Weben_US
dc.titleCharacterization of the sperm chromatin structure in Mytilus californianus and Spisula solidissimaen_US
dc.typeThesisen_US

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