The Identification and characterization of highly immunogenic internal antigens of African trypanosomes

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dc.contributor.authorTuckey, Corinna Dawnen_US
dc.date.accessioned2024-08-15T20:10:34Z
dc.date.available2024-08-15T20:10:34Z
dc.date.copyright1996en_US
dc.date.issued1996
dc.degree.departmentDepartment of Biochemistry and Microbiology
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractSix monoclonal antibodies previously derived against internal Trypanosoma brucei rhodesiense ViTat 1.1 procyclic culture form antigens were characterized for species- and life cycle stage-specificity by immunoblotting. Five of the mAbs were specific for T. brucei spp. while the sixth (mAb #20) recognized an antigen in all trypanosome species and life cycle stages tested. Immunogold electron microscopy revealed that mAb #20 immunoreactivity localized in the mitochondrion. The antigen recognized by mAb #20 was partially purified and the N-terminal sequence showed significant identity with T. cruzi Hsp 60 and E. coli GroEL mitochondrial chaperones. Using a combination of expression library screening and polymerase chain reaction amplification, I isolated the cDNA encoding the trypanosome antigen. The translated sequence matched sequences of mitochondrial chaperones from several species. Taken together, the results indicate that the antigen recognized by mAb #20 is the T. brucei spp Hsp60 mitochondrial chaperone. The mAb #20 or the Hsp60 protein may be used in a simple immunodiagnostic test for African sleeping sickness.
dc.format.extent110 pages
dc.identifier.urihttps://hdl.handle.net/1828/19945
dc.rightsAvailable to the World Wide Weben_US
dc.titleThe Identification and characterization of highly immunogenic internal antigens of African trypanosomesen_US
dc.typeThesisen_US

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