Characterization of genes of stress-related proteins in conifers : low molecular weight heat shock proteins in douglas-fir (Pseudotsuga menziesii [Mirb.] Franco)

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dc.contributor.authorKaukinen, Karia Hannahen_US
dc.date.accessioned2024-08-14T18:21:56Z
dc.date.available2024-08-14T18:21:56Z
dc.date.copyright1996en_US
dc.date.issued1996
dc.degree.departmentDepartment of Biochemistry and Microbiologyen_US
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractTwo cDNA clones (PM 18.2A; PM 18.2B) from Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco), which encode low molecular weight heat shock proteins (LMW HSPs) of 18.2 kDa. were characterized by Northern and Western blot analyses. A notable induction of LMW HSP transcripts occurred during post-gennination and early seedling growth. Unlike late embryogenesis abundant (LEA) protein genes, the expression of the PM HSPs appears to be primarily restricted to seedlings because mRNA transcripts were only detected at very low amounts during seed development and desiccation. The expression of LMW HSP genes in seedlings was shown to be regulated environmentally and hormonally. The LMW HSP proteins assemble into aggregates. The lack of coordinated gene expression of the PM LMW HSPs and HSP 90 suggests specific roles for the LMW HSPs in conifer seedling development. Studies of desiccation proteins in several conifers revealed a diverse array of LENdehydrins during somatic and zygotic embryogenesis and in mature dry seeds. Therefore, these studies suggest a role of stress-related proteins during seed desiccation, germination and growth.
dc.format.extent111 pages
dc.identifier.urihttps://hdl.handle.net/1828/18360
dc.rightsAvailable to the World Wide Weben_US
dc.titleCharacterization of genes of stress-related proteins in conifers : low molecular weight heat shock proteins in douglas-fir (Pseudotsuga menziesii [Mirb.] Franco)en_US
dc.typeThesisen_US

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