Monitoring Metabolic Alteration in Cancer Cells upon Radiation Therapy by Surface-Enhanced Raman Scattering (SERS)

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dc.contributor.authorChen, Xiangyu
dc.contributor.supervisorBrolo, Alexandre Guimaraes
dc.date.accessioned2023-05-31T23:29:08Z
dc.date.available2023-05-31T23:29:08Z
dc.date.copyright2023en_US
dc.date.issued2023-05-31
dc.degree.departmentDepartment of Chemistry
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractSurface-Enhanced Raman Spectroscopy (SERS) is a highly selective and sensitive method that allows the tracking and identification of metabolites at low concentrations in cellular models. In this work, radiation-induced metabolism changes were investigated by SERS using the MCF-7 breast cancer cell line. SERS analysis was carried out on a time course (1, 4, 8, 12-days post exposure) of supernatant samples from MCF-7 cell line cultures previously exposed to either 5 or 20 Gy of ionizing radiation. A significant radiation biomarker, hypoxanthine, was identified through principal component analysis. The amount of hypoxanthine released into the extracellular environment increased over time after exposure to ionizing radiation. A potential mechanism for hypoxanthine production was suggested. Additionally, Caspase-3 inhibitor (Peptide Z-DEVD-FMK) treatment was employed to improve MCF-7 cell viability following exposure to ionizing radiation and to establish the relationship between the release of hypoxanthine and cell apoptosis. This study provides a valuable preliminary assessment of the application of SERS to study metabolic changes that can be extended in the future to human-derived samples.en_US
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/15151
dc.languageEnglisheng
dc.language.isoenen_US
dc.rightsAvailable to the World Wide Weben_US
dc.subjectSurface- Enhanced Raman Scatteringen_US
dc.subjectRaman spectroscopyen_US
dc.subjectMCF-7 cell lineen_US
dc.titleMonitoring Metabolic Alteration in Cancer Cells upon Radiation Therapy by Surface-Enhanced Raman Scattering (SERS)en_US
dc.typeThesisen_US

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