Requirements for ARS2 in RNA processing and retina development

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dc.contributor.authorO'Sullivan, Connor
dc.contributor.supervisorHoward, Perry
dc.date.accessioned2016-09-02T22:26:37Z
dc.date.available2016-09-02T22:26:37Z
dc.date.copyright2016en_US
dc.date.issued2016-09-02
dc.degree.departmentDepartment of Biochemistry and Microbiology
dc.degree.levelDoctor of Philosophy Ph.D.en_US
dc.description.abstractARS2 is a stable component of the nuclear cap-binding complex (CBC) and is critical for RNA Polymerase II transcript processing. As such, ARS2 functions in numerous RNA Polymerase II transcript processing events, which happen co-transcriptionally from initiation to termination, and post-transcriptionally during maturation and export into the cytoplasm. Developmentally, ARS2 is essential for stem cell maintenance and differentiation during embryogenesis and in neural stem cells. Two major questions in the field were: 1) how does ARS2 function in stem cell maintenance and/or differentiation? and 2) how does ARS2 distinguish between disparate RNA classes and processing complexes? In chapter 2, I show that ARS2 is required for the proliferation and cell fate decisions of progenitors in the mouse retina. Specifically, ARS2 knockdown delays cell cycle progression and leads to premature cell cycle exit. Additionally, ARS2 knockdown increases the proportion of cells expressing rod photoreceptor marker Nrl, and decreases Müller glial marker expression. Similarly, knockdown of FLASH, an essential component for replication-dependent histone transcript processing and cell cycle progression, increases the proportion of cells expressing the Nrl reporter, suggesting ARS2’s role in histone processing is contributing to cell cycle progression and fate specification in the developing retina. In chapter 3, I used bioinformatics analysis and homology modeling to classify four structural domains of mammalian ARS2, including a newly identified RNA recognition motif (RRM), and performed mutagenesis to assess their functions. The unstructured C-terminus is required for interaction with the CBC, the Mid domain is implicated in binding DROSHA, which is required for microRNA biogenesis, while the zinc finger and RRM are involved in binding FLASH. Moreover, the zinc finger is required for interacting with RNA. Collectively, this work establishes a model where ARS2 acts as a scaffold, using multiple domains to interact with distinct processing complexes in a mutually exclusive manner. It is also the first study describing the requirements of ARS2 in the developing retina. Understanding the molecular mechanisms governing progenitor proliferation and cell fate specification is crucial in order to design therapies for retinal degenerative diseases.en_US
dc.description.proquestcode0487en_US
dc.description.proquestemailconnoro@uvic.caen_US
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/7521
dc.language.isoenen_US
dc.rightsAvailable to the World Wide Weben_US
dc.rights.urihttp://creativecommons.org/licenses/by-nd/2.5/ca/*
dc.subjectCap-binding complexen_US
dc.subjectRetina developmenten_US
dc.subjectReplication-dependent histone RNAen_US
dc.subjectMicroRNAen_US
dc.subjectCell cycleen_US
dc.subjectCell fateen_US
dc.subjectRNA Polymerase IIen_US
dc.titleRequirements for ARS2 in RNA processing and retina developmenten_US
dc.typeThesisen_US

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