Differences in Soil Fungal Communities between Forested Reclamation and Forestry Sites in the Alberta Oil Sands Region
Date
2023
Authors
Trofymow, John A.
Shay, Philip-Edouard
Tomm, Bradley
Bérubé, Jean A.
Ramsfield, Tod
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Fungi
Abstract
Fungi play key roles in forest soils and provide benefits to trees via mycorrhizal symbioses. After severe disturbance, forest regrowth can be impeded because of changes in fungal communities. In 2013–2014, soil fungi in forest floor and mineral soil were examined by Roche 454 pyrosequencing in undisturbed, harvested, and burned jack pine stands in a forested area near Fort Chipewyan, Alberta. These fungal communities were compared with jack pine, white spruce, and larch stands in Gateway Hill, a nearby certified reclaimed area. In 2014, a more detailed sampling of forestry and reclamation jack pine sites examined fungi in soil fractions using two high-throughput sequencing platforms and a sporocarp survey. The significances of compositional and functional differences in fungal communities between the forested and reclamation sites were assessed using permutation tests of partially constrained ordinations, accounting for confounding factors by variance partitioning. Taxa associated with the forestry area were primarily ectomycorrhizal. Fungal richness and diversity were greater in soils from the reclamation sites and included significantly more pathogenic taxa and taxa with unknown functional properties. Fungal community dissimilarities may have been artefacts of historical legacies or, alternatively, may have resulted from contrasting niche differentiation between forestry and reclamation sites.
Description
Keywords
Illumina, pyrosequencing, sporocarp survey, internal transcribed spacer, jack pine, boreal forest
Citation
Trofymow, J. A., Shay, P.-E., Tomm, B., Bérubé, J. A., & Ramsfield, T. (2023). Differences in soil fungal communities between forested reclamation and forestry sites in the Alberta Oil Sands Region. Journal of Fungi, 9(11), 1110. https://doi.org/10.3390/jof9111110