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Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells

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dc.contributor.author Meghan, Robinson
dc.contributor.author Sarah, Douglas
dc.contributor.author Stephanie, Michelle Willerth
dc.date.accessioned 2017-08-29T16:36:56Z
dc.date.available 2017-08-29T16:36:56Z
dc.date.copyright 2017 en_US
dc.date.issued 2017
dc.identifier.citation Robinson, M., Douglas, S. & Willerth, S. (2017). Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells. Scientific Reports, (7), 1-9. en_US
dc.identifier.uri http://dx.doi.org/10.1038/s41598-017-06570-9
dc.identifier.uri https://dspace.library.uvic.ca//handle/1828/8485
dc.description.abstract Recent work demonstrated that 3D fibrin scaffolds function as an effective substrate for engineering tissues from pluripotent stem cells. However, the rapid degradation rate of fibrin remains a major limitation when differentiating human pluripotent stem cells for tissue engineering applications. The addition of crosslinking agents, such as genipin, during the polymerization process increases scaffold stability while decreasing the degradation rate of fibrin. Genipin crosslinking alters the physical characteristics of the fibrin scaffolds, which influences the behaviour of the differentiating cells seeded inside. It also possesses neuritogenic and neuroprotective properties, making it particularly attractive for engineering neural tissue from pluripotent stem cells. Here we show that genipin enhances neuronal differentiation of neural progenitors derived from human induced pluripotent stem cells (hiPSCs) in 2D culture and genipin concentration influences the morphological and mechanical properties of 3D fibrin scaffolds. These mechanically stable genipin-crosslinked fibrin scaffolds support hiPSC-derived neural aggregates and induce neurite outgrowth while remaining intact for 2 weeks as opposed to 5 days for unmodified fibrin scaffolds. en_US
dc.description.sponsorship This work was supported with funding from the NSERC Discovery Grant Program, the Stem Cell Network, and the Canada Research Chairs program (S.M.W.). The scanning electron microscopy was performed at the Advanced Microscopy Facility at the University of Victoria. en_US
dc.language.iso en en_US
dc.publisher Scientific Reports en_US
dc.rights Attribution 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ca/ *
dc.title Mechanically stable fibrin scaffolds promote viability and induce neurite outgrowth in neural aggregates derived from human induced pluripotent stem cells en_US
dc.type Article en_US
dc.description.scholarlevel Faculty en_US
dc.description.reviewstatus Reviewed en_US


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