A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects

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dc.contributor.authorThorsen, Jim
dc.contributor.authorZhu, Baoli
dc.contributor.authorFrengen, Eirik
dc.contributor.authorOsoegawa, Kazutoyo
dc.contributor.authorde Jong, Pieter J
dc.contributor.authorKoop, Ben F
dc.contributor.authorDavidson, William S
dc.contributor.authorHøyheim, Bjørn
dc.date.accessioned2014-07-14T20:15:55Z
dc.date.available2014-07-14T20:15:55Z
dc.date.copyright2005en_US
dc.date.issued2005-04-04
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: As farming of Atlantic salmon is growing as an aquaculture enterprise, the need to identify the genomic mechanisms for specific traits is becoming more important in breeding and management of the animal. Traits of importance might be related to growth, disease resistance, food conversion efficiency, color or taste. To identify genomic regions responsible for specific traits, genomic large insert libraries have previously proven to be of crucial importance. These large insert libraries can be screened using gene or genetic markers in order to identify and map regions of interest. Furthermore, large-scale mapping can utilize highly redundant libraries in genome projects, and hence provide valuable data on the genome structure. Results: Here we report the construction and characterization of a highly redundant bacterial artificial chromosome (BAC) library constructed from a Norwegian aquaculture strain male of Atlantic salmon (Salmo salar). The library consists of a total number of 305 557 clones, in which approximately 299 000 are recombinants. The average insert size of the library is 188 kbp, representing 18-fold genome coverage. High-density filters each consisting of 18 432 clones spotted in duplicates have been produced for hybridization screening, and are publicly available [1]. To characterize the library, 15 expressed sequence tags (ESTs) derived overgos and 12 oligo sequences derived from microsatellite markers were used in hybridization screening of the complete BAC library. Secondary hybridizations with individual probes were performed for the clones detected. The BACs positive for the EST probes were fingerprinted and mapped into contigs, yielding an average of 3 contigs for each probe. Clones identified using genomic probes were PCR verified using microsatellite specific primers. Conclusion: Identification of genes and genomic regions of interest is greatly aided by the availability of the CHORI-214 Atlantic salmon BAC library. We have demonstrated the library's ability to identify specific genes and genetic markers using hybridization, PCR and fingerprinting experiments. In addition, multiple fingerprinting contigs indicated a pseudo-tetraploidity of the Atlantic salmon genome. The highly redundant CHORI-214 BAC library is expected to be an important resource for mapping and sequencing of the Atlantic salmon genomeen_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by grants from the Research Council of Norway, NFR 139617/140 Salmon Genome Project, NFR 145847/140 The construction of an Atlantic salmon (Salmo salar) BAC library. This work was also supported by grant from Genome Canada (Genome Research on Atlantic Salmon Project).en_US
dc.identifier.citationThorsen J. et al. A highly redundant BAC library of Atlantic salmon (Salmo salar) BMC Genomics 2005, 6 :50en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2164/6/50
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-6-50
dc.identifier.urihttp://hdl.handle.net/1828/5455
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biology
dc.titleA highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projectsen_US
dc.typeArticleen_US

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